安徽农业科学2017，Vol.45Issue(30)：46-48,50,4.
组培固定番茄杂种优势优化体系的建立
Optimization System of Fixed Tomato Heterosis by Plant Tissue Culture
摘要
Abstract
[Objective]To establish optimization system of fixed tomato heterosis by plant tissue culture.[Method]Taking lateral bud from out-standing hybrid tomato plants as explant, MS culture medium as the base,to screen out the induction and enrichment medium for adventitious bud by addition of different ratios of 6-BA and IAA.Meanwhile, to screen out the rooting medium by adding different concentrations of NAA.[Re-sult] By static culturing at the temperature of 25-26 ℃ with illumination at 3000 lx for 12 h/d,the testing explant can be inducted to a well growing adventitious bud in the culture medium containing MS+6-BA 2.4 mg/L+IAA 0.60 mg/L.This adventitious bud also can be massively enriched.Rooting status showed well in 1/2MS culture medium with NAA at the concentration of 0.5 mg/L.[Conclusion] The optimization sys-tem of fixed tomato heterosis by plant tissue culture can massively and quickly enrich the lateral bud of tomato with heterosis as tissue cultured seedlings, which can be used for fixing the heterosis of tomato and speeding up the cultivation of new tomato species.关键词
番茄/植物组织培养/固定/杂种优势Key words
Tomatoes/Plant tissue culture/Fixed/Heterosis分类
农业科技引用本文复制引用
高玉蓉,刘振国,和江明,李卫芬,朱莉..组培固定番茄杂种优势优化体系的建立[J].安徽农业科学,2017,45(30):46-48,50,4.基金项目
国家蔬菜改良中心云南分中心资助项目[农办计(2015) 69号] （2015）
云南省科技惠民项目"茄果类(辣椒、番茄、茄子)蔬菜新品种选育与示范"(2014RA057). （辣椒、番茄、茄子）
