安徽农业科学2017，Vol.45Issue(30)：122-124,3.
基于ITS2序列的丹参及其混伪品分子鉴定研究
Molecular Identification of Salvia Miltiorrhiza Bge.and Its Adulterants Based on ITS2 Sequences
张萍 1黎先军 2杜微波 1陈敬然 1付静 1张志强 1沈建梅1
作者信息
- 1. 北京康仁堂药业有限公司,北京101301
- 2. 天津红日药业股份有限公司,天津301700
- 折叠
摘要
Abstract
[Objective] The research aimed to study the molecular identification of Salvia miltiorrhiza and its adulterants by DNA barcode and specific primers PCR.[ Method] The ITS2 sequence was used as DNA barcoding, and the materials were amplified by PCR and sequenced, and the NJ phylogenetic tree was constructed.The secondary structure of ITS2 was predicted by database established and its website by Koetschan et al.,and the self-designed primers were used to carry out specific primer PCR identification.[ Result] ITS2 sequence length was around 470 bp.The results of cluster analysis showed that Salvia miltiorrhiza and its adulterants were clustered in different branches and showed single character.Compared with secondary structure, Salvia miltiorrhiza and Ganxi rat tail had little difference, and there were signifi-cant difference on the number, size, location of burdock in the spiral stem, and the rotation angle of the spiral arm from the central ring with burdock.The specific primers could distinguish the Salvia miltiorrhiza and its counterfeit by PCR technique.[Conclusion]DNA barcoding and specific primers PCR are effective in distinguishing Salviae miltiorrhiza and its adulterants, and it has an important application foreground in the identification of Chinese herbal medicines.关键词
丹参/DNA条形码/二级结构/PCR技术Key words
Salvia Miltiorrhiza Bge./DNA barcoding/Secondary structure/PCR technique分类
医药卫生引用本文复制引用
张萍,黎先军,杜微波,陈敬然,付静,张志强,沈建梅..基于ITS2序列的丹参及其混伪品分子鉴定研究[J].安徽农业科学,2017,45(30):122-124,3.
