安徽农业科学2017，Vol.45Issue(30)：131-133,168,4.
山羊诱导性多能干细胞培养体系的筛选
Selection on the Culture System of Goat-induced Pluripotent Stem Cells
摘要
Abstract
[Objective]A culture system suitable for goat-induced pluripotent stem cells (iPSCs) was screened by using combination of various culture components.[Method]With the lentivirus as vector carrying pluripotency factor,goat iPSCs were cultured with a variety of culture compo-nents ( culture medium,serum and cytokine) to optimize the culture system.[ Result] The forming efficiency and clone quality of clones could be improved by FBS instead of KSR.The forming efficiency and clone quality of iPSCs clones were not improved by DMEM /F12 instead of KNOCK-OUT-DMEM.The culture system withβ-FGF could maintain the undifferentiated state of iPSCs,while only LIF could only maintain the undifferen-tiated state of iPSCs in the culture system.[Conclusion]Goat iPSCs were successfully induced and cultured in vitro.关键词
诱导性多能干细胞/重编程/培养体系Key words
Induced pluripotent stem cells/Reprogramming/Culture system分类
农业科技引用本文复制引用
宋辉,李卉,王锋..山羊诱导性多能干细胞培养体系的筛选[J].安徽农业科学,2017,45(30):131-133,168,4.基金项目
宁夏自然科学基金项目(NZ15071). （NZ15071）
