安徽农业科学2017，Vol.45Issue(30)：134-139,211,7.
盐芥EsABI1基因克隆及其功能预测
Cloning of EsABI1 of Eutrema salsugineum and Its Function Prediction
摘要
Abstract
[Objective]To clone the EsABI1 of Eutrema salsugineum and predict the function.[Method]The cDNA sequence of EsABI1 of E.sal-sugineum was cloned to study the protein conserved domain and phyletic evolution.[Result]EsABI1 and AtABI1 had highest similarity and be-longed to one branch in evolution,which contained 11 conserved domains,including PP2C protein phosphatase catalytic active site.Quantitative PCR detection showed that the expression level of EsABI1 in EsABI1 silent plants was lower than that in the wild type.The expression of EsABI1 had been up-regulated under 60μmol/L ABA for 0,3,6 hours in E.salsugineum,and the higher up-regulated expression of EsABI1 compared with wide type.[Conclusion] EsABI1 acted as a negative regulator in ABA signal transduction pathway of E.salsugineum,which participated in the re-sponse of E.salsugineum to environmental stress.关键词
盐芥/EsABI1/ABA/基因沉默/基因表达Key words
Eutrema salsugineum/EsABI1/Abscisic acid/Gene silencing/Gene expression分类
农业科技引用本文复制引用
谷彩红,赵宝添,高世庆,陈家红,张荃..盐芥EsABI1基因克隆及其功能预测[J].安徽农业科学,2017,45(30):134-139,211,7.基金项目
山东省自然科学基金项目(ZR2014CM041) （ZR2014CM041）
北京市自然科学基金项目(6162009). （6162009）
