北京中医药大学学报2017，Vol.40Issue(9)：729-735,7.DOI:10.3969/j.issn.1006-2157.2017.09.005
脾虚1号方对功能性消化不良脾虚证大鼠胃体组织MLC蛋白与基因表达的影响
Influence of Pixu Fang 1 (Spleen-deficiency Formula 1) on protein and gene expressions of myosin light chain in rats with functional dyspepsia-spleen deficiency pattern
摘要
Abstract
Objective To investigate the intervention of Pixu Fang 1 (Spleen-deficiency Formula 1) to the protein and mRNA expressions of myosin light chain (MLC) in gastric tissue in rats with functional dyspepsia (FD)-spleen deficiency pattern(SDP).Methods SD rats (n =60,aged 7 d) were randomly divided into normal group (n =10) and FD-SDP model group (n =50).The normal group was orally given 2％ sugar solution and FD-SDP model group was given 0.1％ iodoacetamide sugar solution in a dose of O.2 mL each rat a day for 6 d.The FD-SDP model group was fed with normal forage until rats aged 6 weeks and additionally given modified multiple platform method (MMPM) continuously for 14 d.After modeling,FD-SDP model group was divided randomly into model group (distilled water,10 mL/kg · d),domperidone group (3.125 mg/kg · d),low-dose Pixu Fang 1 group (low-dose group,1.275 g/ kg · d),mid-dose Pixu Fang 1 group (mid-dose group,2.550 g/kg · d) and high-dose Pixu Fang 1 group (high-dose group,5.100 g/kg · d,each n =10),and all groups were given orally corresponding medicinal for 14 d.The protein and mRNA expressions of MLC in gastric tissue were detected by using immunohistochemistry technique,Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal group,MLC protein expression decreased in model group detected by immunohistochemistry technique and Western blotting,and MLC mRNA expression decreased in model group detected by RT-PCR (P ＜0.01).Compared with model group,MLC protein expression increased in low-dose group,mid-dose group and high-dose group detected by immunohistochemistry technique (P ＜ 0.05,P ＜ 0.01).MLC protein expression increased in mid-dose group and high-dose group detected by Western blotting (P ＜ 0.O1).MLC mRNA expression increased in high-dose group detected RT-PCR (P ＜ 0.01).Conclusion There are decreases of protein and mRNA expressions of MLC in FD-SDP rats,and Pixu Fang 1 can up-regulate these expressions.关键词
脾虚1号方/功能性消化不良/脾虚证/胃动力/肌球蛋白轻链/大鼠Key words
Pixu Fang 1 (Spleen-deficiency Formula 1)/functional dyspepsia/spleen deficiency pattern/gastric dynamics/myosin light chain/rats分类
医药卫生引用本文复制引用
吕林,王凤云,唐旭东,尹晓岚,石啸双,段园志..脾虚1号方对功能性消化不良脾虚证大鼠胃体组织MLC蛋白与基因表达的影响[J].北京中医药大学学报,2017,40(9):729-735,7.基金项目
国家重点基础研究发展计划(973计划)资助项目(No.2013CB531703),国家自然科学基金资助项目(No.81503567),中国博士后科学基金资助项目(No.2015M1227,2016T90195)National Key Basic Research Development Plan(973 Plan,No.2013CB531703),National Natural Science Foundation of China(No.81503567),China Postdoctoral Science Foundation (No.2015M1227,2016T90195) （973计划）
