北京中医药大学学报2017，Vol.40Issue(9)：736-742,7.DOI:10.3969/j.issn.1006-2157.2017.09.006
培元解郁方对几种抑郁模型小鼠的抗抑郁作用及机制研究
Anti-depressant effect and mechanism of Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula) in several mouse models of depression
摘要
Abstract
Objective To study the anti-depressant effect and mechanism of Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula).Methods The reserpine depression model was established by intraperitoneal injection of reserpine (2.5 mg/kg) in mice,acute stress depression model,by forced swimming test (FST) in mice,and chronic stress depression model,by long-term bandage (14 d) and noise in mice.The influences of Peiyuan Jieyu Fang on body temperature,ptosis and rate of overstepping bound in reserpine depression model,and on immobility time of FST in acute stress depression model and chronic stress depression model were observed.The content of brain 5-hydroxytryptamine (5-HT),noradrenalin (NA) and indoleamine-2,3-dioxygenase (IDO) in acute stress depression model,and content of 5-HT and IDO in chronic stress depression model were detected by using enzyme-linked immunosorbent assay (ELISA).The expression of IDO mRNA was detected by using fluorescence quantitative polymerase chain reaction (PCR) in chronic stress depression model.Results The decrease of body temperature was antagonized in all Peiyuan Jieyu Fang groups (abbreviated as high-dose group,mid-dose group and low-dose group) in reserpine depression model (P ＜ 0.01).The ptosis and akinesia induced by reserpine were relieved in high-dose group (P ＜ 0.05),and akinesia was relieved in low-dose group (P ＜ 0.05).The immobility time of FST was shortened (P ＜ 0.05,P ＜ 0.01),and content of 5-HT and NA were significantly increased in acute stress depression model in mid-dose group and low-dose group (P ＜0.05,P ＜0.01).The immobility time of FST was shortened in varying degrees,content of 5-HT was increased,content of IDO was decreased and IDO mRNA expression was up-regulated in chronic stress depression model in high-dose group,mid-dose group and low-dose group (P ＜ 0.05).Conclusion Peiyuan Jieyu Fang has some relief effects on depression symptoms induced by reserpine,FST and chronic stress in mice.The mechanism may be related to that Peiyuan Jieyu Fang can increase the content of 5-HT and NA,inhibit IDO activity,and down-regulate IDO mRNA expression.关键词
培元解郁方/抗抑郁/机制/5-羟色胺/去甲肾上腺素/吲哚胺-2,3-双加氧酶/小鼠Key words
Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula)/anti-depressant/mechanism/5-hydroxytryptamine/noradrenalin/indoleamine-2,3-dioxygenase/mice分类
医药卫生引用本文复制引用
刘保秀,王萍萍,杨桃,李丽娜,李建志,畅洪昇..培元解郁方对几种抑郁模型小鼠的抗抑郁作用及机制研究[J].北京中医药大学学报,2017,40(9):736-742,7.基金项目
国家自然科学基金资助项目(No.81373584)National Natural Science Foundation of China (No.81373584) （No.81373584）
