北京中医药大学学报2017，Vol.40Issue(9)：743-749,7.DOI:10.3969/j.issn.1006-2157.2017.09.007
杞红方对寒凝血瘀衰老大鼠心脏保护作用的实验研究
Heart protective effect of Qihong Fang (Wolfberry and Hawthorn Decoction) in aging rats with cold-congealing and blood-stasis pattern
摘要
Abstract
Objective To discuss the influence of Qihong Fang (Wolfberry and Hawthorn Decoction) on heart in aged rats with cold-congealing and blood-stasis pattern.Methods SD rats (n =48) were divided randomly into normal control group,model group,vitamin E group (13.5 mg/kg),low-dose Qihong Fang group (45 mg/kg),mid-dose Qihong Fang group (90 mg/kg) and high-dose Qihong Fang group (180 mg/kg).The aged rat model of cold-congealing and blood-stasis pattern was established through 28-d ozone leading to aging and 14-d ice water leading to cold-congealing and blood-stasis pattern in other 5 groups except of control group.On the 29th d,the blood samples were collected from abdominal aorta for isolating serum and plasma,and heart was separated too.The vitalities of serum total superoxide dismutase (T-SOD),Cu-Zn SOD,Mn-SOD,catalase (CAT),glutathione peroxidease (GSH-PX),content of malondialdehyde (MDA),and prothrombin time (PT),activated partial thromboplastin time (APTT),thrombin time (TT) and fibrinogen (FIB) were detected.The morphologic changes of heart cells were observed after HE staining,fiber deposition quantity was observed after Masson staining,expression of transforming growth factor β1 (TGF-β1) was detected by using immunohistochemistry technique for reviewing aging model of cold-congealing and blood-stasis pattern,and protective effect of Qihong Fang on heart was observed in rats.Results Compared with normal control group,the vitalities of T-SOD,Cu-Zn-SOD,Mn-SOD,CAT and GSH-PX decreased significantly (P ＜ 0.05),MDA content increased significantly (P＜ 0.05,PT and APTT decreased significantly (P ＜ 0.05),and FIB content increased significantly (P ＜0.05) in model group.After Masson staining,morphometry observation by light microscope showed deposition of heart collagen fibers increased significantly (P ＜ 0.05),and expression of TGF-β1 increased significantly (P ＜ 0.05).Compared with control group,the vitalities of T-SOD,Cu-Zn-SOD,CAT and GSH-PX increased significantly (P ＜ 0.05),MDA content decreased significantly (P ＜ 0.05),PT and ATPP increased significantly (P ＜ 0.05),FIB content decreased significantly (P ＜ 0.05),deposition of heart collagen fibers decreased significantly (P ＜0.05),and expression of TGF-β1 decreased significantly (P ＜ 0.05) in mid-dose and high-dose Qihong Fang groups.Conclusion Qihong Fang has a protective effect on heart in aged rats with cold-congealing and blood-stasis pattern.关键词
杞红方/寒凝血瘀/衰老/心脏/转化生长因子-β1/大鼠Key words
Qihong Fang (Wolfberry and Hawthorn Decoction)/cold-congealing and blood-stasis/senility/heart/transforming growth factor-β1/rats引用本文复制引用
张睿,王玥琦,冯玄烨,王湘,刘琳琳,戴宁,冯丽莉,孙燕,赵欣,蔡大勇..杞红方对寒凝血瘀衰老大鼠心脏保护作用的实验研究[J].北京中医药大学学报,2017,40(9):743-749,7.基金项目
教育部重点研究基金资助项目(No.108019)Key Research Foundation from Ministry of Education (No.108019) （No.108019）
