北京中医药大学学报2017，Vol.40Issue(9)：750-757,8.DOI:10.3969/j.issn.1006-2157.2017.09.008
超声导入复方三七消痛软膏对兔膝骨性关节炎滑膜形态和软骨细胞caspase-9、XIAP表达的影响
Influences of sonophoresis of Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment on synovial morphology and expressions of chondrocyte caspase-9 and XIAP in rabbits with knee osteoarthritis
摘要
Abstract
Objective To observe the influences of sonophoresis of Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment on changes of synovial morphology and expressions of chondrocyte caspase-9 and X-linked inhibitor of apoptosis protein (XIAP) in rabbits with knee osteoarthritis (KOA).Methods New Zealand white rabbits (n =32,6 months old) were randomly divided into group of sonophoresis of Fufang Sanqi Xiaotong (sonophoresis group),group of coupling agent ultrasound treatment (coupling agent group),model group and normal group (each n =8).Except of normal group,other groups received anterior cruciate ligament transaction (ACLT).After 6 weeks,sonophoresis group and coupling agent group were given corresponding therapies for 2 treatment courses and model group and normal group were not given any interventions.The changes of synovial histomorphology were observed with light microscope after HE staining,and expressions of caspase-9 and XIAP were detected by using immunohistochemistry technique in all groups after 2 treatment courses.Results The degeneration degree of synovial membrane under light microscope showed an ascending order in all groups as follows:normal group,sonophoresis group,coupling agent group and model group.The value of integral optical density (IOD) of caspase-9 was higher,and IOD value of XIAP was lower in sonophoresis group,coupling agent group and model group than those in normal group.The IOD value of caspase-9 was lower,and IOD value of XIAP was higher in sonophoresis group and coupling agent group than those in model group.The IOD value of caspase-9 was lower,and IOD value of XIAP was higher in sonophoresis group than those in coupling agent group.Conclusion The sonophoresis of Fufang Sanqi Xiaotong is superior to ultrasound treatment,and has the protective effect on cartilage and relief effect on synovial inflammation,and can delay KOA development.The mechanism may be related to decrease of caspase-9 expression and increase of XIAP expression.关键词
超声导入/复方三七消痛软膏/膝骨性关节炎/天冬氨酸蛋白水解酶-9/X-连锁凋亡抑制蛋白/兔Key words
sonophoresis/Fufang Sanqi Xiaotong (Compound Notoginseng Pain-relieving) Ointment/knee osteoarthritis/caspase-9/X-linked inhibitor of apoptosis protein/rabbits分类
医药卫生引用本文复制引用
樊思琴,李珊,梁杰,林如辉,罗庆禄..超声导入复方三七消痛软膏对兔膝骨性关节炎滑膜形态和软骨细胞caspase-9、XIAP表达的影响[J].北京中医药大学学报,2017,40(9):750-757,8.基金项目
国家自然科学基金资助项目(No.81273774)National Natural Science Foundation of China (No.81273774) （No.81273774）
