北京中医药大学学报2017，Vol.40Issue(9)：764-771,8.DOI:10.3969/j.issn.1006-2157.2017.09.010
零陵香挥发性成分的SPME-GC/MS分析
Volatile constituents of Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil) analyzed by applying solid phase microextraction-gas chromatography-mass spectrometry
摘要
Abstract
Objective To analyze the volatile constituents of Linglingxiang (Lysimachia foenumgraecum,Hance,Holy Basil) by applying solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC/MS).Methods The heads of solid phase microextraction (SPME) with 4 kinds of different coating,75 μm CAR/PDMS,65 μm DVB/PDMS,85 μm PA and 100 μm PDMS,were used to extract the under the best extraction condition,and then these volatile constituents were detected by using gas chromatography-mass spectrometry (GC-MS).Results The key parameters of SPME for extracting volatile constituents from Linglingxiang were as follows:temperature was 85℃C and extracting time was for 50 min.A total of 10 types including 103 kinds of volatile constituents were identified by 4 different SPME extraction heads,and phenols,esters and hydrocarbons were main chemical types of volatile compounds in Linglingxiang.There were totally 6 volatile constituents detected by 4 different SPME extraction heads,and they were Sugarlactone,Dihydroactinidiolide,(+)-cedrol,phytone,phenanthrene,and 3-Amino-4,5-dimethyl-2 (5h)-furanone.Among all extraction heads,the head of 75 μm CAR/PDMS detected the largest number of volatile constituents reached to 43.Conclusion SPME-GC/MS was used for the first time form determining the volatile constituents in Linglingxiang.The method is simple,rapid and accurate,which can offer reference to further studies on the material base of Linglingxiang.关键词
零陵香/挥发性成分/固相微萃取/气相色谱-质谱联用Key words
Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil)/volatile constituents/solid phase microextraction/gas chromatography-mass spectrometry分类
医药卫生引用本文复制引用
唐雪阳,秦优,陈林,周融融,邵湘宁,施树云,唐纯玉,张水寒..零陵香挥发性成分的SPME-GC/MS分析[J].北京中医药大学学报,2017,40(9):764-771,8.基金项目
国家中医药管理局中医药公共卫生专项(No.财社[2011]-76),湖南省中医药研究院院级科研课题(No.201601) Special Project of Public Health of State Adiministration of Traditional China Medicine(Financial Society,No.[2011]-76),Hunan Academy of Chinese Medicine (No.201601) （No.财社[2011]-76）
