北京中医药大学学报2017，Vol.40Issue(9)：772-776,5.DOI:10.3969/j.issn.1006-2157.2017.09.011
响应面法优化白屈菜总生物碱提取工艺
Technique optimization for extracting the total alkaloids from Baiqucai (Herba Chelidonii, Greater Celandine) by response surface analysis
摘要
Abstract
Objective To optimize the best technique for extracting the total alkaloids from Baiqucai (Herba Chelidonii,Greater Celandine).Methods The yield of the total alkaloids was taken as review index by using acid dye colorimetry,and Plackett-Burman design (PBD) test was applied at first for screening 3 main factors influencing the technique of extracting the total alkaloids from Baiqucai and they were ethanol volume fraction,solid-liquid ratio and extracting times.Then the best technologic conditions for extracting the total alkaloids from Baiqucai were determined by applying response surface analysis (RSA) of Box-Behnken design (BBD) test.Results The best extracting technique was as follows:ethanol volume fraction was 85％,extracting duration was 60 min,extracting temperature was 70℃,degree of grinding was 40 meshes,solid-liquid ratio was 1:15,and extracting times were 3 times.In these conditions,the yield of the total alkaloids from Baiqucai was up to 1.92％,which was basically the same as theoretical prediction (1.95％).Conclusion The extracting technique optimized in this test is reasonable and feasible,and can get higher content of the total alkaloids.关键词
白屈菜/总生物碱/Plackett-Burman设计/Box-Behnken设计/提取工艺Key words
Baiqucai (Herba Chelidonii, Greater Celandine)/total alkaloids/Plackett-Burman design/Box-Behnken design/extracting technique分类
医药卫生引用本文复制引用
姜华,李军,李欣,董海鹏,石可愿,刘丹丹..响应面法优化白屈菜总生物碱提取工艺[J].北京中医药大学学报,2017,40(9):772-776,5.基金项目
河南省自然科学基金项目(No.162300410104),河南科技大学博士科研启动基金项目(No.13480058)Natural Science Foundation of Henan Province of China (No.162300410104) （No.162300410104）
