北京中医药大学学报2017，Vol.40Issue(9)：777-782,6.DOI:10.3969/j.issn.1006-2157.2017.09.012
清热化湿祛瘀法对慢性肾衰竭湿热证患者瘦素介导的JAK/STAT信号通路的影响
Influence of heat-clearing dampness-resolving stasis-dispelling therapy on JAK/STAT signaling pathway mediated by Leptin in patients with chronic renal failure-damp-heat pattern
摘要
Abstract
Objective To observe the influences of Qingshen (Kidney-Clearing) Granules,with effects of clearing heat,resolving dampness and dispelling stasis,on expressions of Janus-activated kinase 2 (JAK2) and signal transducers and activator of transcriptions 3 (STAT3) in Janus-activated kinasesignaltransducer and activator of transcription (JAK/STAT) signaling pathway mediated by Leptin in patients with chronic renal failure (CRF)-damp-heat pattern,and investigate the mechanism of Qingshen Granules in treatment of CRF.Methods The patients with CRF-damp-heat pattern (n =68) were randomly divided into treatment group and control group (each n =34).The study was completed actually in 60 patients,and 29 in treatment group and 31 in control group.All 2 groups were given basic treatment of Western medicine,and treatment group was additionally given Qingshen Granules (thrice daily and 1 bas every time) for 12 weeks.The clinical efficacy and changes of serum creatinine (SCr),estimated glomerular filtration rate (eGFR),serum Leptin,JAK2 and STAT3 were observed in 2 groups.Results The clinical efficacy was 86.21％ (25/29) and total effective rate of TCM patterns was 82.76％ (24/29) in treatment group,and 58.06％ (18/31) and 54.84％ (17/31) in control group (P ＜0.05).After treatment,the level of SCr decreased significantly (P ＜ 0.01) and level of GFR increased significantly (P ＜ 0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P ＜ 0.05).After treatment,the level of Leptin,and expressions of JAK2 and STAT3 decreased significantly (P ＜ 0.01) in treatment group compared with those before treatment,which were superior to those in control group at the same term (P ＜ 0.05 or P ＜0.01).Conclusion Qingshen Granules can reduce the level of Leptin and expressions of JAK2 and STAT3,inhibit the activation of JAK/STAT signaling pathway mediated by Leptin,relieve clinical symptoms and improve kidney function in patients with CRF-damp-heat pattern.关键词
慢性肾衰竭/湿热证/清热化湿祛瘀法/清肾颗粒/血清瘦素/Janus激酶2/信号转导和转录激活因子3Key words
chronic renal failure/damp-heat pattern/heat-clearing dampness-resolving stasis-dispelling therapy/Qingshen (Kidney-Clearing) Granules/serum Leptin/Janus-activated kinase 2/signal transducers and activator of transcriptions 3分类
医药卫生引用本文复制引用
王东,王亿平,余敏,胡顺金,吕勇,程皖,刘家生,魏玲,金华..清热化湿祛瘀法对慢性肾衰竭湿热证患者瘦素介导的JAK/STAT信号通路的影响[J].北京中医药大学学报,2017,40(9):777-782,6.基金项目
国家自然科学基金资助项目(No.81473673,No.81403372),安徽省自然科学基金资助项目(No.1508085MH198)National Natural Science Foundation of China (No.81473673,No.81403372),Natural Science Foundation of Anhui Province (No.1508085MH198) （No.81473673,No.81403372）
