大连工业大学学报2017，Vol.36Issue(5)：318-322,5.
圆红冬孢酵母类胡萝卜素合成突变菌株的筛选
Screening of carotenoids mutants in Rhodosporidium toruloides
摘要
Abstract
A mutant strain of oil synthesis , defective strain or carotenoid was constructed by mutagenesis of plasma and guanidine .It is determined that the pathway of lipid synthesis is the dominant branching pathway of acetyl coenzyme A node through the distribution of acetyl coenzyme A flux .Compound mutagenesis was carried out using plasma and nitroso guanidine .XP-1 ,XO-3 ,XY-4 and XR-2 mutants were screened out through ARTP and NTG mutagenesis with 95% and 90% lethal rate .The ability of producing lipid ,composition of fatty acid and content of carotenoid determination results showed that the carotenoid contents of XP-1 ,XO-3 ,XY-4 and other three strains were lower than that in strain NP11 , the metabolic pathway of carotenoids have been destroyed , but the decreasing of carotenoid could not promote the increasing of lipid synthesis .The carotenoid content increased with the decreasing of lipid content in strain XR-2 ,which could reached to 0 .77 μg/g .The results indicated that acetyle-CoA is a weakly rigid node ,and the pathway of lipid synthesis is the dominant branch of the pathway . The reduction of its products can increase the production of carotenoid synthesis .关键词
圆红冬孢酵母/等离子诱变/亚硝基胍诱变/油脂/类胡萝卜素/弱刚性节点Key words
Rhodosporidium toruloides/plasma mutation/nitrosoguanidine mutation/lipid/carote-noid/w eakly rigid node分类
轻工纺织引用本文复制引用
张超蕾,张素芳,刘冰南,王际辉..圆红冬孢酵母类胡萝卜素合成突变菌株的筛选[J].大连工业大学学报,2017,36(5):318-322,5.基金项目
国家自然科学基金项目(31370554) （31370554）
辽宁省科技计划项目(2014211003) （2014211003）
辽宁省教育厅创新团队项目(LT2014010) （LT2014010）
大连市科技计划项目(2014B11NC088). （2014B11NC088）
