大连工业大学学报2017，Vol.36Issue(5)：328-332,5.
不同pH下瘤胃液中纤维素降解产氢菌群的性能
Properties of cellulose-degrading and hydrogen-producing bacteria flora in rumen fluid at different pH
摘要
Abstract
A stable and efficient degradation community was developed based on different initial pH to improve the degradation rate of cellulose and study types and function of bovine rumen microorganisms .The structure and function of bacteria were analyzed by PCR-DGGE .The result showed that the utilization rate of fermentation broth with 5 g/L microcrystalline cellulose as carbon source could reach to the maximum of 81 .3% .The hydrogen production at pH 6 .5 could reach to 178 .2 mL/L .Its degradation rate and hydrogen production were higher than those reported in cellulose degrading bacteria .The community structure analysis showed that it was basically stable after 4 times of domestication . The community is consist of cellulolytic bacteria such as Ruminococcaceae , hydrogen producers such as Anaero f ilum , Enterococcus , Clostridium and Enterobacter cloacae producing H2 from cellulose .关键词
纤维素降解/瘤胃液/产氢菌群Key words
cellulose degradation/rumen fluid/hydrogen-producing bacteria flora分类
生物科学引用本文复制引用
张璐,邢德峰,蒋青青,孙睿,任南琪..不同pH下瘤胃液中纤维素降解产氢菌群的性能[J].大连工业大学学报,2017,36(5):328-332,5.基金项目
国家自然科学基金项目(31470233). （31470233）
