大连工业大学学报2017，Vol.36Issue(5)：333-337,5.
油酸对C57/BL6 J小鼠脂肪干细胞体外成脂分化的影响
Effect of oleic acid on adipogenic differentiation of C57/BL6J mice adipose-derived stem cells in vitro
摘要
Abstract
Adipose-derived stem cells (ADSCs) from C57/BL6J mice were primary cultured and cell surface antigen markers were identificated by flow cytometry . ADSCs were treated with different concentration of oleic acid for 3 d . The morphological changes of ADSCs were observed and the differentiation level was assayed by oil red O staining and cellular triglyceride content was detected to confirm the degree of adipogenic differentiation .The cells increased in volume and lipid droplets in cytoplasm became round after stimulating for 3 d at different oleic acid concentrations .The cellular TG content was significant higher in oleic acid-treated cells than that in control group . The cells treated with oleic acid augmented cellular TG content in a dose dependent manner . The results suggested that oleic acid could promote adipogenic differentiation of C 57/BL6J mice adipose-derived stem cells in v itro .关键词
油酸/脂肪干细胞/成脂分化Key words
oleic acid/adipose-derived stem cells/adipogenic differentiation分类
轻工纺织引用本文复制引用
张靖伟,姚英俊,梁媛,王晗,王际辉..油酸对C57/BL6 J小鼠脂肪干细胞体外成脂分化的影响[J].大连工业大学学报,2017,36(5):333-337,5.基金项目
国家自然科学基金项目(31371764,31370554) （31371764,31370554）
辽宁省自然科学基金项目(2014026016). （2014026016）
