分析测试学报2017，Vol.36Issue(9)：1081-1086,6.DOI:10.3969/j.issn.1004-4957.2017.09.004
新型聚合物多孔涂层毛细管开管柱的制备及电色谱研究
Preparation of a Novel Polymer Porous Layer Open Tubular Capillary Column and Its Electrochromatographic Study
莫荣珍 1徐树娟 1金灿 1王伟 1季一兵1
作者信息
- 1. 中国药科大学理学院,江苏南京210009
- 折叠
摘要
Abstract
A novel polymer porous layer stationary phase containing the copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate (GMA-EDMA) was fabricated,and applied as a porous layer open tubular capillary (PLOT) column.To obtain a suitable capillary for electrochromatography,various conditions including the polymerization time,the ratio of n-propanol/1,4-butanediol and GMA/EDMA in the polymerization mixture were optimized.Scanning electron microscopy demonstrated a nearly uniform polymer porous layer on the inner wall of the open tubular capillary column.In the capillary electrochromatographic mode,the PLOT column provided the reasonable separation of neutral,acidic and basic small molecules based on a reversed phase mechanism.After immobilizing the chiral selector human serum albumin (HSA),the HSA-PLOT column performed a better chiral recognition for five pairs of enantiomers compared with the single-layer polymer coated open tubular capillary column modified with HSA.The intra-day,inter-day and column-to-column relative standard deviations for alkylbenzenes separated on the PLOT column were less than 1.7％,4.8％ and 7.8％,respectively.关键词
多孔涂层毛细管开管柱/毛细管电色谱/人血清白蛋白/手性分离Key words
porous layer open tubular capillary column/capillary electrochromatography/human serum albumin(HSA)/chiral separation分类
化学化工引用本文复制引用
莫荣珍,徐树娟,金灿,王伟,季一兵..新型聚合物多孔涂层毛细管开管柱的制备及电色谱研究[J].分析测试学报,2017,36(9):1081-1086,6.
