分析测试学报2017，Vol.36Issue(9)：1087-1092,6.DOI:10.3969/j.issn.1004-4957.2017.09.005
光谱法与共焦荧光成像法研究罗丹明B与ct-DNA的相互作用
Study on Interaction between Rhodamine B and Calf Thymus DNA by Spectroscopy and Confocal Imaging
摘要
Abstract
The interaction between Rhodamine B and calf thymus DNA (ct-DNA) at physiological conditions were studied by the techniques of absorption spectroscopy,fluorescence spectroscopy and confocal imaging.The results of spectroscopy experiments suggested that the hypochromicity of Rhodamine B could appear in the presence of ct-DNA from 450 nm to 650 nm in the absorption spectroscopy,and the fluorescence of Rhodamine B was quenched in the presence of ct-DNA from 560 nm to 660 nm in the fluorescence emission spectroscopy.At the same time,the fluorescence polarization of Rhodamine B varied with the addition of ct-DNA.It was indicated that Rhodamine B could interact with ct-DNA.In the competition experiments,Rhodamine B was unable to replace methylene blue (MB) from MB-ct-DNA comnplex.It was indicated that the interaction between Rhodamine B and ct-DNA took place by the mode of groove combination.The results of confocal imaging experiments showed that the fluorescence of Rhodanmine B was quenched quite obviously after the addition of ctDNA.HeLa cells were dyed with Rhodamine B and 4',6-diamidino-2-phenylindole (DAPI),then used for confocal imaging.It was further verified that the cell nucleus could be dyed red through the groove combination interaction between Rhodamine B and ct-DNA.关键词
罗丹明B(RB)/小牛胸腺DNA(ct-DNA)/沟槽结合/光谱法/共焦荧光成像Key words
Rhodamine B/ct-DNA/groove combination/spectroscopy/confocal imaging分类
数理科学引用本文复制引用
于博昊,缪文彬,吴婷,康燕,姚建磊,张磊..光谱法与共焦荧光成像法研究罗丹明B与ct-DNA的相互作用[J].分析测试学报,2017,36(9):1087-1092,6.基金项目
国家质检总局科技计划项目(2016IK227) （2016IK227）
