分析化学2017，Vol.45Issue(11)：1686-1693,8.DOI:10.11895/j.issn.0253-3820.170300
基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用
Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol
摘要
Abstract
A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-( 3-dimethylaminopropyl ) carbodiimide / N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0. 1 μg / L, with a working range of 0. 1 - 100 μg / L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93. 3% to 97. 9% with relative standard deviations of less than 7% .关键词
氯霉素/量子点微球/免疫层析试纸条Key words
Chloramphenicol/Quantum dot submicrobeads/Immunochromatographic test strip引用本文复制引用
丁乔棋,李丽,范文韬,吕亚楠,胡建华,闫丽萍,宋素泉..基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用[J].分析化学,2017,45(11):1686-1693,8.基金项目
本文系国家重点研发计划(Nos. 2016YFD0501200, 2016YFD0501009)、中央高校基本业务费( No. Y0201500195)、江苏省自然基金面上项目 (No. BK20161452)、广东省科技计划重点项目(No. 2017B020202010)和江苏高校优势学科建设工程资助项目资助 This work was supported by the National Key R&D Program (Nos. 2016YFD0501200, 2016YFD0501009), the Fundamental Research Funds for the Central Universities (No. Y0201500195), the Natural Science Foundation of Jiangsu Province (No. BK20161452), the Key Program of Science and Technology Planning of Guangdong Province, China ( No. 2017B020202010), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China) . （Nos. 2016YFD0501200, 2016YFD0501009）
