分析化学2017，Vol.45Issue(11)：1727-1733,7.DOI:10.11895/j.issn.0253-3820.171120
新疆紫草的毛细管电泳指纹图谱研究
Capillary Electrophoresis Fingerprint of Arnebia Euchroma (Royle) Johnst.
摘要
Abstract
A method of capillary electrophoresis fingerprint was developed for evaluation of the quality of Arnebia euchroma ( Royle) Johnst. The samples were separated on a 50 μm × 40 cm uncoated capillary separation column at separation voltage of 25 kV with 100 mmol/ L borate buffer ( pH 8. 0) containing 25 mmol/ L SDS and 20% (V/ V) dehydrated alcohol as running buffer. The injection volume of sample was 0. 5 psi ×5 s and the detection wavelength was 214 nm. The results indicated that the samples of Arnebia euchroma (Royle) Johnst were well separated and detected in 35 min. With Shikonin peak as reference peak, 6 characteristic peaks of standard Arnebia euchroma (Royle) Johnst were determined. The quality discriminant analyses were accomplished for different kinds of samples named Arnebia euchroma ( Royle) Johnst that purchased from eight sources by means of characteristic fingerprint peak analysis, similarity evaluation and cluster analysis. This method had good reproducibility, and could be used for the quality control of Arnebia euchroma (Royle) Johnst.关键词
指纹图谱/新疆紫草/毛细管电泳/质量控制Key words
Fingerprint/Arnebia euchroma (Royle) Johnst/Capillary electrophoresis/Quality control引用本文复制引用
张蕾,张爱芹,王嫚,申刚义..新疆紫草的毛细管电泳指纹图谱研究[J].分析化学,2017,45(11):1727-1733,7.基金项目
本文系国家自然科学基金项目(Nos. 81573834、81001595)、国家科技支撑计划项目(No. 2014BAC15B04) 和中央民族大学一流大学一流学科项目(No. YLDX01013)资助 This work was supported by the National Natural Science Foundation of China ( Nos. 81573834, 81001595), the National Science and Technology Support Program, China (No. 2014BAC15B04) and Minzu University of China of the World First-class University and the First-class Discipline (No. YLDX01013). （Nos. 81573834、81001595）
