福州大学学报（自然科学版）2017，Vol.45Issue(5)：731-735,741,6.DOI:10.7631/issn.1000-2243.2017.05.0731
鲍鱼内脏β-葡萄糖苷酶中试规模制备研究
Study on the pilot-plant scale preparation of β-glucosidase from abalone viscera
摘要
Abstract
Theβ-glucosidase from abalone visceral was separated,purified through a pilot scale technique combination including homogenation in colloid mill,centrifugation in high speed tubular centrifuge,filtration upon ceramic composite membrane,ultrafihration,anion-exchange chromatography of DEAE-agarose and cation-exchange chromatography of CM-agarose.It was demonstrated that the total enzyme activity in the homogenate was 12 174.21 U and the specific activity was 0.158 U · mg-1 as well as the specific activity of crude enzyme in the supernatant obtained by 2 times of high speed tubular centrifugation was 0.247 U · mg-1.It was demonstrated that the specific activity of permeate from ceramic composite membrane filtration and concentrate from ultrafiltration were O.133 U · mg-1 and 0.249 U ·mg-1,respectively.Through the study on the elution pH gradient in anion exchange chromatography of DEAE-agarose and cation exchange chromatography of CM-agarose,it was also demonstrated that the best elution pH was about 7.5 and 6.0.The specific activities of β-glucosidase collected from anion-exchange chromatography of DEAE-agarose and cation-exchange chromatography of CM-agarose were 0.492 U · mg-1 and 1.709 U · mg-1,respectively,with a final purification fold was 10.78,and the vield was 5.0％.关键词
鲍鱼内脏/β-葡萄糖苷酶/中试/纯化Key words
abalone viscera/β-glucosidase/pilot scale/purification分类
生物科学引用本文复制引用
陈胜男,张利,石贤爱,陈金梅,蔡振辉..鲍鱼内脏β-葡萄糖苷酶中试规模制备研究[J].福州大学学报（自然科学版）,2017,45(5):731-735,741,6.基金项目
国家海洋公益性行业科研专项子课题(201205022-3) （201205022-3）
