影像科学与光化学2017，Vol.35Issue(6)：788-796,9.DOI:10.7517/j.issn.1674-0475.2017.05.001
D60K Mb定点突变对肌红蛋白结构及荧光量子产率的影响
Research on the Effect of Asp60Lys on the Structure and Fluorescence Quantum Yield of Myoglobin
摘要
Abstract
To study the role of surface-charged Asp60 in the fluorescence intensity,under simulative physiological conditions of animal body,the difference in structure and fluorescence spectral characteristics between Mb(WT) and Mb(D60K) was studied by fluorescence spectroscopy and circular dichroism.The results indicated that the fluorescence quantum yield of (D60K) was far more than that of Mb,which may correlate with pH and the structure of Mb.With a further research,this phenomenon was caused by the change of external charge when amino acid Asp60 of myoglobin was replaced by Lys,which enhanced the polarity and exposure extent of aromatic amino acids of myoglobin,and the decay process of myoglobin molecules which were excited from non-radiative decay to radiative decay.关键词
肌红蛋白/D60K Mb/光谱/结构/荧光量子产率Key words
myoglobin/mutant(D60k)/spectroscopy/structure/fluorescence quantum yield引用本文复制引用
唐乾,李如玉,职秋雁,曹洪玉,王立皓,郑学仿..D60K Mb定点突变对肌红蛋白结构及荧光量子产率的影响[J].影像科学与光化学,2017,35(6):788-796,9.基金项目
国家自然科学基金项目(21571025,21271036,21601024)资助 （21571025,21271036,21601024）
