影像科学与光化学2017，Vol.35Issue(6)：853-860,8.DOI:10.7517/j.issn.1674-0475.2017.05.011
量子点-卟啉纳米复合体系在肿瘤细胞成像中的应用
Application of CdTe QDs-TMPyP Nanocomplex in Imaging of Tumor Cells
摘要
Abstract
The solution CdTe quantum dots (Dots) with high quantum yield and good homogeneous distribution conjugate meso-tetrakis (N-methylpyridinium-4-yl) porphyrin (TMPyP) to construct QDs-TMPyP nanocomplex.The QDs-TMPyP nanocomplex was achieved to detection of DNA by photo induced electrontransfer (PIET) process.CdTe QDs and CdTe QDs-TMPyP showed a remarkable inhibition for the proliferation of cancer cells in vitro at higher concentrations,but only small effects at lower concentrations.The cells viabilities of HeLa cells were above 92％ under treated with lower than 1.0 μmol/L CdTe QDs and CdTe QDs-TMPyP for 24 h.The HeLa cancer cells treated with 0.2 μmol/L CdTe QDs-TMPyP were found to contain Cd and Te atoms by EDS analysis.The uptake of CdTe QDs-TMPyP was easier than CdTe QDs in HeLa cancer cells.The CdTe QDs-TMPyP nanocomplex was successfully achieved in HeLa cells fluorescent imaging,which lays the foundation for the drug delivery and cell imaging.关键词
量子点/α,β,γ,δ-四(1-甲基吡啶嗡-4-基)卟吩对甲苯磺酸盐/荧光成像/DNAKey words
quantum dots/TMPyP/fluorescent imaging/DNA引用本文复制引用
胡先运,孟铁宏,黄星宏,张汝国,江家志,刘瑛,朱楠..量子点-卟啉纳米复合体系在肿瘤细胞成像中的应用[J].影像科学与光化学,2017,35(6):853-860,8.基金项目
国家自然科学基金项目(21545006)、贵州省自然科学基金(2015GZ48861,2016GZ13752)、中央高校基本科研业务费专项资金(KYLX0187)、黔南州优青项目、黔南科技计划项目(黔南科合工字[2016]12号,黔南科合社字[2016]1号)和黔南民族医专科研基金(QNYZ201601,QNYZ201605)资助 （21545006）
