广西植物2017，Vol.37Issue(10)：1270-1278,9.DOI:10.11931/guihaia.gxzw201703014
褐纹报春苣苔组织培养与快速繁殖
Tissue culture and rapid propagation of Primulina glandaceistriata
摘要
Abstract
Primulina glandaceistriata is a highly ornamental value of the wild flowers in karst area, yet there is no re-search on the tissue culture of P. glandaceistriata. We took the leaves of P. glandaceistriata as material to study tissue culture and rapid propagation by two methods. The results showed that the optimum medium for adventitious bud regener-ation was MS+6-BA 2.0 mg·L-1+NAA 0.10 mg·L-1, the optimum medium for multiplication culture was MS+ZT 2.0 mg·L-1+NAA 0.10 mg·L-1+ active carbon 0.05 g·L-1 and the multiplication coefficient was 11.09, the optimum cul-ture medium which induced the callus was MS+TDZ 2.0 mg·L-1+NAA 0.10 mg·L-1, the best differentiation mediumwas MS+ZT 1.0 mg·L-1+NAA 0.10 mg·L-1 and the differentiation coefficient was 12.46, the optimum medium for roo-ting culture was 1/2MS+IBA0.1 mg·L-1+ active carbon 0.05 g·L-1 and 1/2MS+IBA0.5 mg·L-1+ active carbon 0.05 g·L-1 , the rooting rate was 100%. In this study, tissue culture and rapid propagation system of P. glandaceistriata was successfully developed to provide a theoretical basis and technical support for future research on genetic transformation.关键词
苦苣苔/褐纹报春苣苔/组织培养/不定芽/愈伤组织Key words
Gesneriaceae/Primulina glandaceistriata/tissue culture/adventitious buds/callus分类
农业科技引用本文复制引用
闫海霞,邓杰玲,黄昌艳,关世凯,何荆洲,张自斌,卜朝阳..褐纹报春苣苔组织培养与快速繁殖[J].广西植物,2017,37(10):1270-1278,9.基金项目
广西科学研究与技术开发计划项目(桂科攻1598006-5-8,桂科能1598022-1-5) （桂科攻1598006-5-8,桂科能1598022-1-5）
南宁市科学研究与技术开发计划项目(NC20152008-3) （NC20152008-3）
广西农科院项目(2015YT89)Supported by the Guangxi Science and Technology Construction Planning(1598006-5-8, 1598022-1-5) （2015YT89）
Nanning Science and Technology Construction Planning(NC20152008-3) （NC20152008-3）
Basic Research Fund of Guangxi Academy of Agri-cultural Sciences(2015YT89). （2015YT89）
