基础医学与临床2017，Vol.37Issue(11)：1535-1540,6.
匹诺塞林缓解大鼠肝细胞系BRL-3A低氧/复氧损伤
Pinocembrin alleviates hypoxia reoxygenation injury in rat liver cell line BRL-3A
摘要
Abstract
Objcetive To investigate protective effect of pinocembrin ( PIN)on hepatocytes induced by hypoxia/reoxygenation ( H/R) as well as its relationship to the TLR 4/NF-κB signaling pathway .Methods The cells were randomly divided into 4 groups: control group, PIN group, hypoxia/reoxygenation injury group and PIN pretreatment group .The cell viability was measured with CCK-8.The apoptosis rate was determined by Annexin V-FITC/PI staining.The activity of ALT was detected .ELISA was used to evaluate the contents of TNF-αand IL-β.The mRNA and protein expression level of TLR 4, IκB-αand NF-κB P65 in cells was observed by quantita-tive real-time PCR or Western blot .Results The H/R stimulation decreased cell survival rate and enhanced the apoptosis .The activity of ALT was increased .The contents of TNF-αand IL-1βwere significantly enhanced , and the expression level of TLR4 and NF-κB P65 was markedly increased while IκB-αdecreased.After pretreatment with PIN, the cell survival rate increased while the apoptosis rate decreased .The activity of ALT was decreased. TNF-αand IL-1βwere reduced significantly and the expression level of TLR 4 and NF-κB P65 were decreased while IκB-αincreased.Conclusions PIN has protective effects on hypoxia/reoxygenation injury, which might be mediated in part by TLR4/NF-κB signaling pathway .关键词
匹诺塞林/低氧/复氧/肝细胞损伤/Toll样受体4/核转录因子-κBP65Key words
pinocembrin/hypoxia/reoxygenation/hepatocyte injury/toll-like recepcor4/nuclear factor-κb P65分类
医药卫生引用本文复制引用
郭振,牟童,李婷婷,朱迪,蒲俊良,吴忠均..匹诺塞林缓解大鼠肝细胞系BRL-3A低氧/复氧损伤[J].基础医学与临床,2017,37(11):1535-1540,6.基金项目
国家自然科学基金(81672959) （81672959）
重庆市科委科技人才培养计划(cstc2015shmszx120019 ) （cstc2015shmszx120019 ）
