基础医学与临床2017，Vol.37Issue(11)：1552-1556,5.
HPV31型L2保守中和表位可诱发广谱中和抗体
Conservative epitope of human papillomavirus type 31 L2 can induce broad-spectrum neutralizing antibodies
摘要
Abstract
Objective To detect the immunogenicity of conservative neutralizing epitope from human papilloma-virus type 31 ( HPV31) minor capsid protein L2, and analyze the neutralizing antibody spectrum of its immune sera.Methods Synthesize HPV31 L2 aa.17-40 peptide and conjugate with KLH by EDC.Immunize New Zeal-and white rabbits with HPV31 L2-KLH and Freud's Adjuvant.Analyze the neutralizing antibody titers of immune sera against HPVs from α4,α7,α9,α10 and β1 species by pseudovirus neutralization assay.Results In rabbits, HPV31 L2-KLH induced broad-spectrum neutralizing antibodies against at least 17 types of HPV, among which the neutralizing antibody titers against HPV31 are the highest, followed by that of HPV5/45/57. Conclusions The broad-spectrum neutralizing activity of the immune sera of HPV31 L2 is stimulated by conser-vative neutralizing epitope.The results lay the foundation of the development of broad-spectrum HPV vaccines based on HPV31 L2 neutralizing epitope.关键词
人乳头瘤病毒31型/L2保守中和表位/广谱中和抗体Key words
human papillomavirus type 31/L2 conservative neutralizing epitope/broad-spectrum neutralizing antibody分类
医药卫生引用本文复制引用
张婷,陈雪,刘洪洋,周艳,王志荣,许雪梅..HPV31型L2保守中和表位可诱发广谱中和抗体[J].基础医学与临床,2017,37(11):1552-1556,5.基金项目
国家国际科技合作项目(2013DFA32430) （2013DFA32430）
中国医学科学院医学与健康科技创新工程(2016-I2M-3-026) （2016-I2M-3-026）
