基础医学与临床2017，Vol.37Issue(11)：1579-1584,6.
脂多糖促进大鼠肝星状细胞系HSC-T6细胞自噬
LPS promotes autophagy in rat hepatic stellate cell line HSC-T6
摘要
Abstract
To investigate the effects of lipopolysaccharide (LPS) on autophagy in rat hepatic stellate cells (HSC-T6) and involvement of NF-κB pathway in it.Methods 1)HSC-T6 cells were treated with LPS at the concentraction of 0, 0.01, 0.1, 1, and 10 mg/L for 0, 3, 6, 12, 18, and 24 h respectively, Microtubule-associated protein light chain Ⅱ (LC3Ⅱ) and Beclin1 levels were detected by Western blot; 2)HSC-T6 cells were randomized into groups of control group, LPS group, PDTC group, LPS+PDTC group, PMA group and LPS+PMA group, Western blot assay was used to measure the levels of LC 3Ⅱand Beclin1, immunofluoresence was used to measure NF-κB P65 intracellular distribution.The levels of hydroxyproline (Hyp) were determined by the method of colorimetry and the levels of laminin (LN) and hyaluronic acid (HA) were determined by ELISA in the culture supernatants after corresponding processing .Results The levels of LC3Ⅱand Beclin1 were significantly increased after HSC-T6 cells were treated with LPS at the concentraction of 0.1 mg/L for 6 h and the levels of Hyp, LN, and HA in the culture supernatants increased remarkably as well (P<0.05).PDTC pretreatment increased the levels of LC3Ⅱand Beclin1 in the LPS-treated HSC-T6 cells while decreased the levels of Hyp , LN, and HA significantly (P<0.05).PMA pretreatmen decreased the levels of LC 3Ⅱand Beclin1 in the LPS-treated HSC-T6 cells while increased the levels of Hyp, LN, and HA (P<0.05).Conclusions LPS can promote autophagy and activation of NF -κB pathway in HSC-T6 cells.Activation of NF-κB pathway may inhibit the LPS-induced autophagy of HSC-T6 cells.关键词
肝星状细胞/核因子-κB/自噬/脂多糖Key words
hepatic stellate cell/NF-κB/autophagy/LPS分类
医药卫生引用本文复制引用
王美娇,徐军全,王明亮,宋彬妤,吴惠文,康杰,刘晓玲..脂多糖促进大鼠肝星状细胞系HSC-T6细胞自噬[J].基础医学与临床,2017,37(11):1579-1584,6.基金项目
山西医科大学汾阳学院科技发展基金( 1303 ) （ 1303 ）
