解放军医学杂志2017，Vol.42Issue(10)：870-875,6.DOI:10.11855/j.issn.0577-7402.2017.10.06
归芪益元膏对12C6+束辐射损伤模型大鼠旁效应的影响
Effect of Guiqiyiyuan ointment on bystander effect in rat models of 12C6+ beam radiation damage
摘要
Abstract
Objective To investigate the bystander effect injury to lung-heart-liver-spleen caused by 12C6+ beam radiation,and explore the prevention and treatment effect of Guiqiyiyuan ointment on the injury and its mechanism.Methods Ninety healthy male Wistar rats were randomly divided into 3 groups:NC group (normal control,normal saline 2ml/kg,n=30),SR group (simple radiation,8Gy,2ml/kg,n=30),GO group (Guiqiyiyuan ointment 11.83g/kg,radiation,8Gy,n=30).All the rats received intragastric administration for 7 days.The right side of the lung was modeled by 12C6+ beam radiation.After modeling,the rats were killed at 48h.The heart,liver and spleen were taken.The malonaldehyde (MDA),glutathione (GSH),glutathione peroxidase (GSH-Px),superoxide dismutase (SOD) contents were measured by colorimetry,DNA methylation rate was assayed by ELISA,and the expressions of Dnmt1,Dnmt3a and Dnmt3b were detected by immunohistochemistry.Results Compared with NC group,the contents of SOD,GSH and GSH-Px decreased (P＜0.01),MDA increased (P＜0.01),the level of DNA methylation decreased (P＜0.01),and the expressions of Dnmt1,Dnmt3a and Dnmt3b increased in SR group (P＜0.01).Compared with SR group,the contents of SOD,GSH and GSH-Px increased (P＜0.01),MDA decreased (P＜0.01),the level of DNA methylation increased (P＜0.01),and the expressions of Dnmt1,Dnmt3a and Dnmt3b decreased in GO group (P＜0.01).Dnmtl,Dnmt3a and Dnmt3b proteins were expressed in the cytoplasm of myocardial cells,hepatocytes and peripheral B cells of the white pulp in spleen,in all the groups.The color of NC group was light brown-brown,showing a weak positive expression.The color of SR group was brown-brown,showing a strong positive expression.The color of GO group was light brown-tan,showing a moderate positive expression.Conclusion The Guiqiyiyuan ointment can reduce the bystander effect caused by the 12C6+ beam radiation,and its mechanism is related to improving the oxidative stress reaction and the level of DNA methylation.关键词
归芪益元膏/辐射损伤/旁效应/氧化应激/DNA甲基化Key words
Guiqiyiyuan ointment/radiation injury/bystander effect/oxidative stress/DNA methylation分类
医药卫生引用本文复制引用
梁建庆,李金田,李娟,刘永琦,蔺兴遥,刘凤艳,安耀荣,李晓伟,张子同..归芪益元膏对12C6+束辐射损伤模型大鼠旁效应的影响[J].解放军医学杂志,2017,42(10):870-875,6.基金项目
国家自然科学基金(81460695) （81460695）
中国博士后科学基金第60批面上资助项目(2016M602887).This work was supported by the National Natural Science Foundation of China (81460695),and the China Postdoctoral Science Foundation(2016M602887) （2016M602887）
