解放军医学杂志2017，Vol.42Issue(10)：887-890,4.DOI:10.11855/j.issn.0577-7402.2017.10.09
存活蛋白对胶质瘤细胞替莫唑胺敏感性的影响
Knockdown of survivin expression increases chemosensitivity of glioma cells to temozolomide
王鹏 1张剑宁 1于新 1刘锐 1陈金辉 1孙艳杰1
作者信息
摘要
Abstract
Objective To study the effect of survivin on temozolomide (TMZ) resistance of glioma cells and possible mechanism.Methods Survivin RNA and survivin shRNA plasmids were transfected into U87 cells,respectively.The cell growth curve drawn by MTT was used to test the changes in the proliferation of glioma cells.Colony forming assay was used to calculate cell surviving fraction.The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining were applied to detect the apoptosis rate by flow cytometer (FCM).Western blotting was used to detect the expression of activated caspase-3.Results The cell proliferation and cell surviving fraction were inhibited by TMZ,and it was more significantly inhibited in TMZ plus down-regulation of survivin.Compared with parental cells,U87/sur si cells,treated by TMZ,showed a decrease in proliferation and colony forming efficiency and an increase in apoptosis rate,whereas U87/sur cells,treated by TMZ,showed an increase in proliferation and colony forming efficiency and a decrease in apoptosis rate.Western blotting showed that compared with TMZ alone,the expression of activated caspase-3 protein was promoted in TMZ plus down-regulation of survivin.Conclusion Down-regulation of survivin could enhance the sensitivity of glioma cells to TMZ,which may be related to survivin promoting cell apoptosis.关键词
胶质瘤/细胞凋亡/替莫唑胺/存活蛋白Key words
glioma/apoptosis/temozolomide/survivin分类
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王鹏,张剑宁,于新,刘锐,陈金辉,孙艳杰..存活蛋白对胶质瘤细胞替莫唑胺敏感性的影响[J].解放军医学杂志,2017,42(10):887-890,4.
