环境与职业医学2017，Vol.34Issue(10)：914-917,4.DOI:10.13213/j.cnki.jeom.2017.17156
氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响
Effects of fluoride exposure on expression of SOST and DKK1 in rat skin fibroblasts
摘要
Abstract
[Objective] To observe the changes of protein expressions of sclerostin (SOST) and dickkopf-1 (DKK1) in fibroblasts of rat skin following fluoride exposure.[Methods] Rat skin fibroblasts were cultured with fluoride at different 0,0.0001,0.001,0.01,0.1,1,10,and 20mg/L.The expression levels of SOST and DKK1 in cell culture supematants were detected at four exposure time points (24,48,72,and 96h) by ELISA.[Results] The expression levels of SOST of the 0.000 1,0.001,0.01 mg/L fluoride groups at 48h,the 1 and 10mg/L fluoride groups at 72 h,and the 0.001,0.01,0.1,and 1 mg/L fluoride groups at 96 h were significantly lower than that of the 0 mg/L fluoride group (P＜0.05).The expression levels of SOST of the 0.01 mg/L fluoride group at 48h and the 0.001,0.01,0.1,1,and 10mg/L fluoride groups at 96h were significantly lower than those of the groups at 24h (P＜0.05).The expression levels of DKK1 of the 0.001 mg/L fluoride group at 48h,the 0.01,0.1,1,10,and 20mg/L fluoride groups at 72h,and the 0.01,0.1,1,10,and 20mg/L fluoride groups at 96 h were significantly lower than that of the 0 mg/L fluoride group (P ＜ 0.05).The expression levels of DKK1 of the 0.0001,0.001,0.01 mg/L fluoride groups at 48 h,the 0.001,0.01,1,10,and 20 mg/L fluoride groups at 72 h,and the 0.01,0.1,1,and 20 mg/L groups at 96 h were significantly lower than those of the groups at 24 h (P ＜ 0.05).[Conclusion] Fluoride can cause down-regulation of expression of SOST and DKK1 in rat skin fibroblasts.关键词
硬骨素/DKK1/成纤维细胞/氟Key words
sclerostin/dickkopf-1/fibroblast/fluoride bone injury分类
医药卫生引用本文复制引用
刘晓利,刘芳盈,孟超,李平,张殿平,宋菁,刘克俭..氟染毒对大鼠皮肤成纤维细胞中SOST及DKK1蛋白表达的影响[J].环境与职业医学,2017,34(10):914-917,4.基金项目
国家自然科学基金项目(编号:81072255、81372941) （编号:81072255、81372941）
