环境与职业医学2017，Vol.34Issue(10)：918-922,5.DOI:10.13213/j.cnki.jeom.2017.17184
高氯酸盐对大鼠免疫功能的影响
Effects of perchlorate on immune function of rats
摘要
Abstract
[Objective] To study the effects of perchlorate on immune function of rats,and understand the immune toxicity of perchlorate.[Methods] Thirty-six healthy Wistar rats (180-200 g) were randomly divided into six groups.Perchlorate was administered by gavage at 4.50,22.00,110.00,and 560.00mg/kg (in terms of body weight),respectively.Distilled water was used as negative control,and cyclophosphamide was used as positive control (intraperitoneal injection).After exposure to perchlorate for continuous 5 d (once a day) and observation for 2 d,rat tail vein blood was collected on the 7th day,and body weight and organ weights were measured to calculate organ coefficients.White blood cell counts and differential counts were calculated by test-tube method.Concentrations of T lymphocyte subset indices (CD4+ and CDs+) and cytokines (IFN-γ,IL-4,and IL-10) were detected by ELISA,and CD4+/CD8+ was also calculated.[Results] Compared with the negative control group,the rat thymus coefficient and spleen coefficient in the perchlorate groups (except the 4.50mg/kg group) decreased significantly (P＜0.05 or P＜0.01) and especially in the 560.00mg/kg group,as well as in the positive control group (P ＜0.01).Compared with the negative control group,neutmphils increased significantly in the groups administered with 22.00 mg/kg perchlorate and above (P＜ 0.01);the concentrations of CD4+ decreased significantly in the 110.00 mg/kg group and 560.00mg/kg group;IL-4 and IL-10 were significantly decreased in the groups administered with 22.00mg/kg perchlorate and above (P＜ 0.05 or P＜0.01).[Conclusion] Perchlorate may suppress immune function of rats.关键词
高氯酸盐/免疫毒性/IFN-γ/CD4+/CD8+Key words
perchlorate/immune toxicity/IFN-Y/CD4+/CD8+分类
医药卫生引用本文复制引用
唐玄乐,韩丹,于佳,史力田,刘晓秋,刘家仁..高氯酸盐对大鼠免疫功能的影响[J].环境与职业医学,2017,34(10):918-922,5.基金项目
黑龙江省教育厅科学技术研究项目(编号:12541388) （编号:12541388）
