棉花学报2017，Vol.29Issue(5)：437-446,10.DOI:10.11963/1002-7807.lhzsj.20170818
陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究
Analysis of the Correlation between Pigment Glands and (-)/(+)-Gossypol Contents in Upland Cotton
摘要
Abstract
[Objective] The aim of this study was to examine the correlation between pigment glands and (-)/(+)-gossypol contents in different organs of different upland cotton genotypes.[Method] Using upland cotton materials CCRI 12,CCRI 12 (glandless),Coker 312,Coker 312 (glandless),IF 11 and Jimian 14,we accurately measured the density and size of the pigment glands,tested (-)/(+)-gossypol contents by pre-column derivatization-high performance liquid chromatography (HPLC),and then analyzed the correlation between pigment glands and (-)/(+)-gossypol contents.[Result] The whole plants of low gossypol cotton lines were without pigment glands,but free gossypol could still be detected in different organs at mass fractions less than 0.02％.The organs of glanded cotton all had typical pigment glands,the gland density was highest in the seed and lowest in the bract.The gland size (diameter) was largest in the boll shell and smallest in the petal and seed.Across different organs,the total gossypol content was highest in the seed and lowest in the bract.The contents of (+)-gossypol in the seed and petal were higher than (-)-gossypol.Conversely,the (-)-gossypol contents of the calyx,boll shell,upper leaf,middle leaf,and Dower leaf were higher than (+)-gossypol.There was a significant negative correlation between the density and size of glands in glanded cotton.There were significant positive correlations between total gossypol content and (-)/(+)-gossypol contents,and between (+)-gossypol content and (-)-gossypol content.Gland density was positively correlated with total gossypol content and (-)/(+)-gossypol contents.Gland size was negatively correlated with (+)-gossypol content,and showed no significant correlation with (-)-gossypol content or total gossypol content.[Conclusion] Overall,the strong positive correlation between total gossypol content in the seed and petal suggests that we can use the petal gossypol content to infer the seed gossypol content.关键词
陆地棉/色素腺体密度/色素腺体大小/左旋棉酚/右旋棉酚/相关性分析Key words
upland cotton/pigment gland density/pigment gland size/(-)-gossypol/(+)-gossypol/correlation analysis分类
农业科技引用本文复制引用
刘慧,季灵艳,赵天伦,陈进红,祝水金..陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究[J].棉花学报,2017,29(5):437-446,10.基金项目
国家重点研发计划(2016YFD0101400) （2016YFD0101400）
国家自然科学基金(31501342) （31501342）
国家现代农业产业技术体系——棉花产业体系岗位科学家专项(CARS-18-25) （CARS-18-25）
江苏省现代作物生产协同创新中心 （）
