四川大学学报（自然科学版）2017，Vol.54Issue(5)：1125-1130,6.DOI:10.3969/j.issn.0490-6756.2017.05.040
甘蓝型油菜独脚金内酯相关基因的克隆与表达分析
Molecular cloning and expression analysis of strigolactones-related genes in Brassica napus L.
摘要
Abstract
Strigolactones(SLs) constitute the recently discovered group of plant hormones,they participate in inhibiting shoot branching.Three SLs-related genes were obtained from Brassica napus by homologous cloning,named BnMAX4,BnMAX1,BnD14;the length of CDS was 1707,1593,804 bp;the putative protein function was β-carotenoid oxygenase,cytochrome P450,α/β hydrolase,respectively.Using roots,stems and leaves of wild type B.napus at two-euphylla one-bud and four-euphylla onebud stage,expression of the three genes was analyzed by RT-qPCR,the results showed that BnMAX4 mainly expressed in the roots at two-euphylla stage;BnMAX1 didn't show expression advantage;BnD14 significantly expressed in leaves at two-euphylla stage.Then expression of the three genes was analyzed under the drought and salt stress,the expression of BnMAX4 suddenly decreased under both stress conditions.The expression of BnMAX1 decreased under drought stress;under salt stress the expression decreased first and increased afterwards,after 9 h decreased again.The expression of BnD14 showed two high peaks at 3 h and 12 h under drought stress;under salt stess the expression showed one high peak at 3 h.关键词
甘蓝型油菜/独脚金内酯/基因克隆/表达分析/旱、盐胁迫Key words
Brassica napus/Strigolactones/Gene cloning/Expression analysis/Drought/salt stress分类
生物科学引用本文复制引用
张璐,宋验红,解艳芳,谢甜,王茂林..甘蓝型油菜独脚金内酯相关基因的克隆与表达分析[J].四川大学学报（自然科学版）,2017,54(5):1125-1130,6.基金项目
国家科技支撑计划(2013BAD01B03) （2013BAD01B03）
四川省十二五油菜育种攻关项目(SN2011yzgg05) （SN2011yzgg05）
国家“863”计划(2011AA10A10401) （2011AA10A10401）
