兽类学报2017，Vol.37Issue(4)：389-398,10.DOI:10.16829/j.slxb.201704009
神农架川金丝猴源肠道粪肠球菌的分离与鉴定
Isolation and identification of intestinal Enterococcus faecalis from golden snub-nosed monkeys in Shennongjia National Natural Reserve
摘要
Abstract
Eight strains with obvious antibacterial effect were isolated from the contents of the intestinal of healthy wild golden snub-nosed monkeys (Rhinopithcus roxellana) in Shennongjia by differential cultivation,morphology observation and antimicrobial tests.They were identificated as Enterococcus faecalis by physiological and biochemical identification,and 16S rRNA sequence analysis.Of which,two good safety strains were screened through the detection of virulence factors and the animal acute toxicity test.Their characteristics of tolerating gastrointestinal environment (low pH,high bile) and growth were examined.The results showed that E.faecalis dlt7a and dlt7b were safe,and probiotic for the body,and they own the features that rapid propagation,a strong ability to withstand the gastric acid and intestinal bile salt.They may be used as candidate probiotics strains for golden snub-nosed monkeys in the future.关键词
金丝猴/粪肠球菌/分离鉴定/益生菌Key words
Enterococcus faecalis/Golden snub-nosed monkeys/Isolation and identification/Probiotics分类
生物科学引用本文复制引用
呙会会,白文妹,李翔,杨敬元,姚辉,肖运才..神农架川金丝猴源肠道粪肠球菌的分离与鉴定[J].兽类学报,2017,37(4):389-398,10.基金项目
国家“十二五”科技支撑计划项目(2013BAD03B01) （2013BAD03B01）
