陕西师范大学学报（自然科学版）2017，Vol.45Issue(5)：30-36,7.DOI:10.15983/j.cnki.jsnu.2017.05.252
碱性磷酸酶定位方向对DPPC液晶单层膜热力学特性的影响
Orientation of alkaline phosphatase change the thermodynamic characteristics of DPPC liquid crystal monolayer
摘要
Abstract
In order to investigate the interaction between alkaline phosphate (AP) and phospholipid monolayer on the molecular level,the effect of alkaline phosphate on the DPPC monolayer was studied by thermodynamics in the three different methods of AP addition.The results showed that the phase transition of DPPC monolayer was not affected by AP in the two manners of co-spreading and subphase formation.At low surface pressure,the major axis of AP molecule turns to be parallel to the interface from vertical.The change of AP's orientation on the DPPC monolayer influences the interaction force between the molecules,reduces the disorder of the monolayer and increases the energy of system.In contrast,in the method of injection,AP molecules make the phase transition of DPPC monolayer become insignificant.The major axis of AP remains to be parallel to the interface at low surface pressure.However,at high surface pressure,AP molecules might be encased by DPPC micelles and released to the outside of monolayer.It not onlychanges the intermolecular interaction force but also increases the disorder of DPPC monolayerand decreases the energy of system.Therefore,the orientation of alkaline phosphatase molecule plays a critical role in its interaction with the DPPC monolayer.关键词
水溶性蛋白/相转移/分子间相互作用力/混乱度/DPPC单层膜Key words
soluble protein/phase transition/intermolecular interaction force/disorder/DPPC monolayer分类
数理科学引用本文复制引用
王娟,孙润广,郝长春,苗宗成,马亚红,李拓,辛督强..碱性磷酸酶定位方向对DPPC液晶单层膜热力学特性的影响[J].陕西师范大学学报（自然科学版）,2017,45(5):30-36,7.基金项目
国家自然科学基金(51673157,11544009) （51673157,11544009）
