山西医科大学学报2017，Vol.48Issue(10)：999-1002,4.DOI:10.13753/j.issn.1007-6611.2017.10.005
附子、黄连寒热药性对体外足细胞增殖及TRPV4、TRPC6蛋白表达的影响
Effect of Fuzi and Huanglian on in vitro cell proliferation and the expression of TRPC6 and TRPV4
摘要
Abstract
Objective To explore the effects of Fuzi and Huanglian on podocyte proliferation and the expression of TRPV4 and TRPC6 proteins in vitro.Methods ①MTT method was used to detect the podocyte proliferation.The experiment was divided into blank control group,Fuzi group and Huanglian group.Different concentrations of Fuzi and Huanglian were used to treat the podocytes.The absorbance value(A) was measured,and the inhibition rate of cells was calculated.②The experiment was divided into blank control group,Fuzi(200 μg/ml) group and Huanglian(800 μg/ml) group.The podocytes were given liquid medicine,and then cultured with RPMI 1640 medium for 24 h to detect the expression of TRPV4 and TRPC6 protein by Western blot.Results MTT results showed that 50,100,200,400 and 800 μg/ml Huanglian inhibited the growth and proliferation of podocyte in a concentration-dependent manner.The relatively low concentration of Fuzi (＜200 μg/ml) promoted the growth and proliferation of podocytes,while the relatively high concentration(＞ 200 μg/ml) inhibited the growth and proliferation of podocytes.The results showed that the expression of TRPV4 protein in Fuzi group and Huanglian group were significantly increased compared with blank control group (P ＜ 0.05),while the expression of TRPC6 protein in Fuzi and Huanglian group were decreased (P ＜ 0.05).Conclusion The traditional Chinese medicine Fuzi and Huanglian all have the influence on the expression of TRPV4 and TRPC6 in kidney disease.关键词
寒热药性/TRPV4/TRPC6Key words
different drug properties/TRPV4/TRPC6分类
医药卫生引用本文复制引用
王竹,孙万森,李欣,李睿萍..附子、黄连寒热药性对体外足细胞增殖及TRPV4、TRPC6蛋白表达的影响[J].山西医科大学学报,2017,48(10):999-1002,4.基金项目
国家自然科学基金资助项目(81072974) （81072974）
