山西医科大学学报2017，Vol.48Issue(10)：1008-1012,5.DOI:10.13753/j.issn.1007-6611.2017.10.007
miR-1202在宫颈癌组织中的表达及对HeLa细胞增殖及凋亡的影响
Expression of miR-1202 in cervical cancer tissues and its effect on proliferation and apoptosis of HeLa cells
摘要
Abstract
Objective To investigate the expression of miR-1202 in human cervical cancer tissues and its effects on proliferation and apoptosis of HeLa cells.Methods The qRT-PCR was used to detect the expression of miR-1202 in 29 cases of cervical cancer sampies and control tissues.MiR-1202 overexpression vector was constructed.MiR-1202 inhibitor was synthesized.MiR-1202 overexpression vector and miR-1202 inhibitor were transfected into HeLa cells.HeLa cells were divided into four groups:control group(miR-control),miR-1202 overexpression vector group(miR-1202),miR-1202 inhibitor negative control group(inhibitor NC) and miR-1202 inhibitor group(miR-1202 inhibitor).MTT assay was used to analyze the effects of miR-1202 on proliferation of cervical cancer HeLa cells.The effects of miR-1202 on the cell cycle and apoptosis of cervical cancer HeLa cells were observed by flow cytometer.Results The expression of miR-1202 significantly down-regulated in cervical cancer tissues(P ＜ 0.01).Compared with miR-control,miR-1202 overexpression vector decreased the OD value (P ＜ 0.01),increased the proportion of G1/G0 phase cells(84.63％ ±5.08％,P ＜ 0.01),reduced the proportion of S and G2/M phase cells (11.56％ ±2.51％ and 4.62％ ± 1.75％,P ＜ 0.01),and increased the proportion of early apoptosis and late apoptosis (19.68 ％ ±2.66％ and 10.89％ ± 1.94％,P ＜ 0.01).Compared with inhibitor NC,miR-1202 inhibitor increased the OD value (P ＜ 0.01),diminished the proportion of G1/G0 phase cells (43.68 ％ ±4.86 ％,P ＜ 0.01),increased the proportion of S and G2/M phase cells (38.94％ ±2.86％ and 18.65％ ± 1.82％,P ＜ 0.01),and reduced the proportion of early apoptosis and late apoptosis(2.06％ ±1.33％ and 2.85％ ±1.22％,P ＜0.01).Conclusion The expression of miR-1202 decreases in cervical cancer tissues,and it can inhibit the proliferation of cervical cancer cells and induce the apoptosis.关键词
miR-1202/宫颈癌/细胞增殖/细胞周期/细胞凋亡Key words
miR-1202/cervical cancer/cell proliferation/cell cycle/cell apoptosis分类
医药卫生引用本文复制引用
姚婷婷,张红娜,高婉,朱康,段钊..miR-1202在宫颈癌组织中的表达及对HeLa细胞增殖及凋亡的影响[J].山西医科大学学报,2017,48(10):1008-1012,5.基金项目
陕西省自然科学基金资助项目(2013JM4012) （2013JM4012）
