深圳大学学报（理工版）2017，Vol.34Issue(5)：471-475,5.DOI:10.3724/SP.J.1249.2017.05471
REGγ对LPS介导的腹膜炎调节作用
Regulation of REGγ on LPS-mediated peritonitis
摘要
Abstract
Mouse peritonitis model is established by LPS peritoneal injection to analyze the roles of proteasome activator REGγ in LPS-mediated peritonitis.Bone marrow chimera mice are generated by adoptive transfer of bone marrow cells.Survival assay is used to research the effect of REGγ deficiency on survival rate.Expression of proinflammatory factors are detected via enzyme-linked immunosorbent assay (ELISA).As compared to the wild-type (WT) mice,the REGγ-knockout (KO) mice shows significantly lower survival rate and lower expression of the proinflammatory factors TNFα,IL-1β and MCP-1.Furthermore,bone marrow from WT and KO mice is isolated and transferred adoptively to X-ray irradiated WT mice to generate WTchimera and KOchimera bone marrow chimera mice.The results suggest that KOchimera mice also manifest obviously lower survival rate and proinflammatory factors expression of TNFα,IL-1β and MCP-1 after peritoneal injection of LPS to WTchimera and KOchimera.Thus,these data suggest that REGγdeficiency in inflammatory cells of hematopoietic origin accelerates LPS-mediated decline of survival rate and suppresses expression of proinflammatory factors TNFα,IL-1β and MCP-1.关键词
腹膜炎/蛋白酶体/过继转移/骨髓嵌合体/造血系统/酶联免疫吸附测定法Key words
peritonitis/proteasome/adoptive transfer/bone marrow chimera/hematopoietic system/enzyme-linked immunosorbent assay分类
医药卫生引用本文复制引用
孙锦霞,黎丽,王瑞,黄钟..REGγ对LPS介导的腹膜炎调节作用[J].深圳大学学报（理工版）,2017,34(5):471-475,5.基金项目
国家自然科学基金资助项目(31401217) （31401217）
中国博士后科学基金资助项目(2014M0560672)National Natural Science Foundation of China (31401217) （2014M0560672）
Postdoctral Science Foundation of China (2014M0560672) （2014M0560672）
