深圳大学学报（理工版）2017，Vol.34Issue(5)：526-531,6.DOI:10.3724/SP.J.1249.2017.05526
DH-PSF三维动态多粒子追踪在活细胞成像的应用
Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging
摘要
Abstract
Multi-particle tracking is one of the most challenging aspects of living cell imaging.We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm,respectively.The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time.Therefore,this system is a very useful tool for studying the intermolecular interaction in living cell.关键词
光学工程/三雏成像/多粒子/点扩展函数/纳米定位/活细胞/动态追踪Key words
optical engineering/three-dimensional imaging/multi-particle/point spread function/nano-positioning/living cell/dynamic tracking分类
生物科学引用本文复制引用
李恒,陈丹妮,于斌,牛憨笨,郭宝平..DH-PSF三维动态多粒子追踪在活细胞成像的应用[J].深圳大学学报（理工版）,2017,34(5):526-531,6.基金项目
国家自然科学基金资助项目(61605127) （61605127）
广东省自然科学基金资助项目(2014A030312008) （2014A030312008）
深圳市科技计划资助项目(JCYJ20150324141711698,JCYJ20160308104404452)National Natural Science Foundation of China (61605127) （JCYJ20150324141711698,JCYJ20160308104404452）
Natural Science Foundation of Guangdong Province (2014A030312008) （2014A030312008）
Shenzhen Science and Technology Research Foundation (JCYJ20150324141711698,JCYJ20160308104404452) （JCYJ20150324141711698,JCYJ20160308104404452）
