天津科技大学学报2017，Vol.32Issue(5)：10-16,7.DOI:10.13364/j.issn.1672-6510.20160043
乌腺金丝桃总黄酮的超声提取及其抗氧化活性
Ultrasonic Extraction of Total Flavonoids from Hypericum attenuatum Choisy and its Antioxidant Activities
摘要
Abstract
Flavonoid is one of major bioactive components in Hypericum attenuatum Choisy.In this study,the conditions for ultrasonic extraction of total flavonoids fromHypericum attenuatum Choisy were optimized with the methods of single factor and response surface to explore the application value ofHypericum attenuatum Choisy.The results showed that the optimal conditions for the extraction of total flavonoids from Hypericum attenuatum Choisy were 500,W ultrasonic power, 60℃ temperature,62%, ethanol concentration,37﹕1,liquid-solid ratio and 32,min extraction time.Under these conditions, the yield of total flavonoids fromHypericumattenuatumChoisy was 6.20%,.Its extract exhibited different scavenging ca-pacities on different radicals,and it has better scavenging capacities on DPPH radical with an IC50,value of 322.58,μg/mL and poor scavenging capacities on ABTS radical with the concentration of 10-500,μg/mL.关键词
乌腺金丝桃/总黄酮/超声提取/响应面法/抗氧化活性Key words
Hypericum attenuatum Choisy/total flavonoids/ultrasonic extraction/reponse surface method/antioxidant activities分类
医药卫生引用本文复制引用
金杜欣,罗学刚,张克勤,张同存..乌腺金丝桃总黄酮的超声提取及其抗氧化活性[J].天津科技大学学报,2017,32(5):10-16,7.基金项目
国家自然科学基金资助项目(31270837) （31270837）
长江学者和创新团队发展计划资助项目(IRT1166) （IRT1166）
