天然产物研究与开发2017，Vol.29Issue(10)：1635-1641,7.DOI:10.16333/j.1001-6880.2017.10.001
一个鼠尾藻异多糖的结构特征及其抗氧化和抗结肠癌细胞增殖作用
Structural Characterization of a Heteropolysaccharide from Sargassum thunbergii and Its Antioxidant and Anti-colon Cancer Cells Proliferation Activity
摘要
Abstract
DEAE-Sepharose CL-6B column chromatography was used to separate and purify a polysaccharide STP-Ⅰ from the algae Sargassum thunbergii.The structural assignment of STP-Ⅰ was performed using Fourier transform infrared spectroscopy (FTIR) analysis,periodate oxidation-smith degradation,methylation analysis and nuclear magnetic resonance (NMR).The repeating unit of STP-Ⅰ was therefore determined.Major sugar residues of STP-Ⅰ were 6-β-D-Glcp and 4-α-D-Galp,other residues including 5-α-L-Araf,2,3-αt-D-Xylp,T-β-D-Xylp and T-β-D-Glcp.The degree of branching (DB) of STP-Ⅰ was 26.7％.On the basis of radical assays,STP-Ⅰ exhibited higher scavenging activities of hydroxyl and superoxide radicals than Vitamin C with EC50 values of 0.15 and 1 mg/mL,respectively.STP-Ⅰ also exhibited the capability of anti-proliferation in human colon cancer cells Caco-2 with IC50 value of 2.56 mg/mL,and cause little loss to common cells.关键词
鼠尾藻/结构分析/抗氧化活性/抗增殖作用Key words
Sargassum thunbergii/structural analysis/antioxidant activity/anti-proliferation capability分类
生物科学引用本文复制引用
曹森,周容,聂开颖,罗巅辉..一个鼠尾藻异多糖的结构特征及其抗氧化和抗结肠癌细胞增殖作用[J].天然产物研究与开发,2017,29(10):1635-1641,7.基金项目
华侨大学中青年教师科研提升资助计划(ZQN-PY316) （ZQN-PY316）
国家自然科学基金(31201314) （31201314）
华侨大学研究生科研创新能力培育项目 （）
