天然产物研究与开发2017，Vol.29Issue(10)：1671-1676,1711,7.DOI:10.16333/j.1001-6880.2017.10.007
泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响
Effects of Modified Rhizoma Alismatis Decoction on Expression of AOP3 in Rats with Hyperlipidemia
摘要
Abstract
To discuss the mechanism of modified Rhizoma Alismatis Decoction on prevention and treatment of hyperlipemia,through investigating hepatic expressions of AQP3 in rats with hyperlipemia.60 Male Sprague-Dawley rats were randomly divided into 6 groups:Control group,Model control group,Simvastatin control group,High-dose of modified Rhizoma Alismatis Decoction group,Middle-dose of modified Rhizoma Alismatis Decoction group,Low-dose of modified Rhizoma Alismatis Decoction group.Rats in control group were fed with ordinary forage,and the others were fed with high-fat forage.At the same time,each treatment group was administrated with appropriate drug.Then TC,TG,HDL-C and LDL-C of serum were detected,the genetic expression of AQP3 in colon tissue were detected with RT-PCR and the protein expression of AQP3 in colon tissue were detected with immunohistochemical means and Western Blot after 5 weeks.In the model control group,the content of TG,TC,LDL-C in serum and the expression of AQP3 in colon tissue were significantly elevated,while the content of HDL-C was significantly reduced.After treatment,the contents of TG,TC,LDL-C and the expression of AQP3 in every treatment groups were reduced,while the contents of HDL-C were elevated.Modified Rhizoma Alismatis decoction had a satisfying effect on regulating the blood lipid.Increasing the genetic and protein expression of AQP3 in colon tissue,regulating the water fluid metabolism,preventing the formation of sputum and removing the pathogenic factors could be viewed as mechanisms of modified Rhizoma Alismatis Decoction concerning prevention and treatment of hyperlipemia.关键词
泽泻汤加味方/高脂血症/水通道蛋白3/实验研究Key words
modified Rhizoma Alismatis decoction/hyperlipidemia/AQP3/experimental research分类
医药卫生引用本文复制引用
王亚芬,韩雪,张一昕,段思明,郝蕾,王茜,刘晨旭..泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响[J].天然产物研究与开发,2017,29(10):1671-1676,1711,7.基金项目
河北省中医药管理局科学技术研究计划(201 50030) （201 50030）
河北省高等学校科学技术研究项目(QN2015251) （QN2015251）
