天然产物研究与开发2017，Vol.29Issue(10)：1688-1694,7.DOI:10.16333/j.1001-6880.2017.10.010
连翘花中抑制酪氨酸酶活性成分研究
Activity-guided Isolation of Mushroom Tyrosinase Inhibitors from the Flower of Forsythia suspensa
摘要
Abstract
This is the first time to investigate the chemical constitutes of Forsythia suspensa flower for tyrosinase-inhibition activity.First,different organs (flower,leaf,green Forsythiae Fructus and ripe Forsythiae Fructus) of F.suspensa were tested for the tyrosinase-inhibition activity,and the F.suspensa flower showed concentration-dependent on the inhibition of tyrosinase activity.Second,different extracts of F.suspensa flower were also tested for the tyrosinase-inhibition activity,and the 95％ ethanol extract displayed the best inhibition of tyrosinase activity.Then,five compounds were obtained from 95％ ethanol extract by the activity-guided isolation method,and their structures were identified as forsythiaside A'(1),rutin (2),(+)-pinoresinolβ-D-glucoside (3),(+)-pinoresinol monomethyl ether β-D-glucoside (4) and (+)-phillyrin (5),while compound 4was reported for the first time from F.suspensa flower.Four of them showed weak inhibition activity against tyrosinase but displayed obvious synergistic effect.The results implied that the 5 compounds were most likely to be the chemical substance foundation of the whitening effect of F.suspensa flower and its inhibitory effect was possibly produced by synergistic effect.关键词
连翘花/提取和分离/酪氨酸酶抑制/化学物质/协同作用Key words
Forsythia suspensa flower/extraction and separation/tyrosinase inhibition/chemical substance/synergistic effect分类
医药卫生引用本文复制引用
白美美,李丹凤,李石飞,张立伟..连翘花中抑制酪氨酸酶活性成分研究[J].天然产物研究与开发,2017,29(10):1688-1694,7.基金项目
山西省自然科学基金(201603D321024 （）
201601D021157) （）
