天然产物研究与开发2017，Vol.29Issue(10)：1695-1699,5.DOI:10.16333/j.1001-6880.2017.10.011
荞麦麸皮多糖的分离纯化及其外貌表征的分析
Purification and Structural Morphology of Buckwheat Bran Polysaccharide
摘要
Abstract
In this work,Buckwheat Bran Polysaccharides (BBP) was extracted by water extraction and alcohol precipitation method.It was further purified by DEAE-52 cellulose column chromatography and Sephadex G-200.The molecular weight of the purified product was determined by gel filtration method,followed by the morphology characterization and component analysis using electron microscopy.The glycosidic bond configuration of the purified product was analyzed by infrared spectroscopy.The results showed that three polysaccharide fractions (BBP-Ⅰ and BBP-Ⅰ and BBP-Ⅲ) were obtained by DEAE-52 cellulose column chromatography and Sephadex G-200 separation and purification and their molecular weights were 1.56 × 104 Da,5.85 × 104 Da,8.45 × 104 Da,respectively.The electron microscopy showed that the surface of BBP was roughness and uneven.There was significant diversity about the appearance of the purified product,which mainly characterized by spherical,lamellar and rod,respectively.IR spectra demonstrated that BBP-Ⅰ and BBP-Ⅲ were alpha glycosidic bond compounds,while BBP-Ⅱ was beta glycosidic bond compound.The differences of polysaccharide components in buckwheat bran would provide an important foundation for further structural research.关键词
荞麦麸皮多糖/分离纯化/外貌表征分析Key words
buckwheat bran polysaccharide/separation and purification/characterization分类
轻工纺织引用本文复制引用
赵鑫帅,张海生,杨淑芳,朱怡霖,薛焕焕..荞麦麸皮多糖的分离纯化及其外貌表征的分析[J].天然产物研究与开发,2017,29(10):1695-1699,5.基金项目
陕西师范大学中央高校基本科研业务费-创新团队项目(GK201601007) （GK201601007）
仁用杏仁精深加工及其产业化关键技术研究与示范(2017NY-151) （2017NY-151）
