天然产物研究与开发2017，Vol.29Issue(10)：1700-1705,6.DOI:10.16333/j.1001-6880.2017.10.012
羊耳菊活性部位提取物在大鼠胆汁中代谢产物的研究
Metabolism of Active Fractions of Inula Cappa in Rat Bile
摘要
Abstract
To establish an ultra-performance liquid chromatography/quadrupale time-of-flight mass (UHPLC/Q-TOF MS/MS) method for analyzing the metabolites of main component in Inula cappa extract in rat bile after oral administration.Cannulas were surgically inserted into the bile duct of SD rats and bile was collected for 12 h after oral administration of active fractions of Ⅰ.cappa at a dose of 100 g/kg of the material.After n-butyl alcohol precipitation treatment,the chemicals and their metabolites in rat bile were identified using UHPLC/Q-TOF MS/MS with selected reaction monitoring mode.The results revealed that twenty-seven metabolites were determined in bile,main metabolites are hydrogenation,methylation and glucuronidation of caffeoylquinic acid.It indicated that the chemicals can occur biotransformation in bile,the metabolic pathways mainly are methylation and demethylation glucuronidation.关键词
羊耳菊/活性部位提取物/UHPLC/Q-TOF MS/MS/胆汁/代谢产物/咖啡酰基奎宁酸Key words
Inula cappa/active fraction/UHPLC/Q-TOF MS/MS/metabolites/caffeoylquinic acid分类
医药卫生引用本文复制引用
巩仔鹏,候靖宇,候佳,伍萍,李月婷,陈思颖,兰燕宇..羊耳菊活性部位提取物在大鼠胆汁中代谢产物的研究[J].天然产物研究与开发,2017,29(10):1700-1705,6.基金项目
国家自然科学基金(81360680) （81360680）
贵州省优青项目(2015-11) （2015-11）
贵州省科技合作计划(2015-7362) （2015-7362）
贵州省中医药管理局项目(QZZY-2015-078) （QZZY-2015-078）
