天然产物研究与开发2017，Vol.29Issue(10)：1706-1711,6.DOI:10.16333/j.1001-6880.2017.10.013
小花棘豆和苦马豆不同萃取相乙酰胆碱酯酶活性及机理探讨
Acetylcholinesterase Activity and Mechanisms of Different Extracts from Oxytropis glabra DC.and Swainsonia salsula (Pall.) DC.
摘要
Abstract
In order to evaluate the activity and potential application of acetylcholinesterase inhibitors from Oxytropis glabra DC.and Swainsonia salsula(Pall.) DC.,the inhibition mechanism and kinetics were investigated in this study.Each extract was prepared using different polarity solvents associated with ultrasonic assisted extraction.In addition,improved Ellmans'colorimetric method was employed to exploit the acetylcholinesterase inhibition activity and kinetics.Results showed that the chloroform extracts of both root and leaf of O.glabra and the leaf of S.salsula demonstrated higher AChE inhibitory activities over other extracts with the IC50 values of 0.67 mg/mL,1.69 mg/mL and 0.17 mg/mL,respectively.The inhibition mechanism of chloroform extracts from leaf of these two plants are both mixed type reversible inhibition.关键词
萃取物/乙酰胆碱酯酶/抑制机理/动力学Key words
extract/acetylcholinesterase/inhibition mechanism/kinetics分类
生物科学引用本文复制引用
王志雁,沈红玲,孟庆艳,张航航,贾旭,刘文杰..小花棘豆和苦马豆不同萃取相乙酰胆碱酯酶活性及机理探讨[J].天然产物研究与开发,2017,29(10):1706-1711,6.基金项目
国家自然科学基金(21365018) （21365018）
新疆生产建设兵团科技攻关项目(2011AB030) （2011AB030）
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BRYR1404) （BRYR1404）
