天然产物研究与开发2017，Vol.29Issue(10)：1766-1772,1758,8.DOI:10.16333/j.1001-6880.2017.10.022
中药组方“回乳康”对回乳期奶牛血清PRL、GH和STAT5含量的影响
Effect of "Hui Ru-kang" Prescription on PRL, GH and STATS Levels in Dairy Cows' Serum during Milk Withdrawal Period
摘要
Abstract
The aims of this study was to explore the effect of "Hui Ru-kang" prescription on serum PRL,GH and STAT5 levels in dairy cow during milk withdrawal period.80 Healthy cows in late pregnancy that gave 15.42 ± 0.71 kg milk per day were used.The cows were randomly divided into four groups:group A,B,C and D with 20 of them in each group.Dry dairy cows' milk by gradually dry milk method and the day when cows began to dry milk was recorded as 0 d.Group A was a control group,did not feed "Hui Ru-kang".Group B,C and D were fed "Hui Ru-kang" 400,500,600 g respectively at 8:00 am from the 1 d to the day that cows withdrawed milk completely.The cows' venous blood was collected from the tail on the day 0,1,3,5,7,9 and 11.In addition,the daily milk production was noted.ELISA was used to evaluate the expression levels of PRL,GH and STAT5.As the result,the milk production of all 4 groups were on the decline during milk withdrawal period.Group A,B,C and D dried milk on 11,7,5 and 5 d respectively.The expression levels of serum PRL,GH and STAT5 were declining in all four groups and the decline trend of group A was slower than other groups,and the expression levels of GH and STAT5 were raised after milk withdrawal period.The change of PRL,GH,STAT5 expression levels and milk yield in group C were not significantly different between these of group D (P ＞ 0.05).The expression levels of PRL,GH,STAT5 and milk yield showed significant and positive correlation among each other (P ＜ 0.01).During milk withdrawal period,feeding 500 g "Hui Ru-kang" would make dairy cows withdraw milk obviously.关键词
回乳期奶牛/中药组方“回乳康”/催乳素/生长激素/信号转导和转录激活因子5Key words
cows during milk withdrawal period/" Hui Ru-kang" prescription/PRL/GH/STAT5分类
农业科技引用本文复制引用
沈留红,肖劲邦,巫晓峰,姜思汛,曹随忠,余树民..中药组方“回乳康”对回乳期奶牛血清PRL、GH和STAT5含量的影响[J].天然产物研究与开发,2017,29(10):1766-1772,1758,8.基金项目
四川省教育厅重点项目(15ZA0024) （15ZA0024）
四川农业大学双支计划(03571537) （03571537）
