天然产物研究与开发2017，Vol.29Issue(10)：1773-1778,1784,7.DOI:10.16333/j.1001-6880.2017.10.023
响应面法优化黑根霉胞外多糖脱蛋白工艺研究
Optimization of Deproteinization Process of Exopolysaccharides from Rhizopusnigricans by Response Surface Methodology
摘要
Abstract
The effects of papain concentration,enzymolysis temperature and time on the deproteinization of extracellular polysaccharide from Rhizopusnigricans in Enzyme-Sevag process were investigated by single-factor experiment combining with central composite design method of Box-Behnken.The optimal process parameters were determined based on the deproteinization rate and comprehensive index.After optimizing by response surface methodology,the optimal process parameters were confirmed as follows:papain concentration of 425 U/mL,enzymolysis temperature of 62 ℃ and time of 90 min.Following repeated experiments three times,the average deproteinization rateand comprehensive index were determined as 54.08 ％ and 2.07,respectively.关键词
黑根霉/胞外多糖/脱蛋白/酶-Sevag法/响应面法Key words
Rhizopusnigricans/exopolysaccharides/deproteinization/enzyme-sevag process/response surface methodology分类
医药卫生引用本文复制引用
秦国正,琚勇,张明芮,李萍,柳春燕,唐保露,薛信磊,王国栋..响应面法优化黑根霉胞外多糖脱蛋白工艺研究[J].天然产物研究与开发,2017,29(10):1773-1778,1784,7.基金项目
安徽高校自然科学研究项目(KJ2016A723,KJ2015A199,KJ2017A257) （KJ2016A723,KJ2015A199,KJ2017A257）
安徽省自然科学基金(1508085MH191) （1508085MH191）
皖南医学院中青年科研基金(WK201517,WK201601,WK201610) （WK201517,WK201601,WK201610）
大学生创新创业训练计划(201510368017,201510368119,201610368120) （201510368017,201510368119,201610368120）
