现代妇产科进展2017，Vol.26Issue(11)：801-805,5.DOI:10.13283/j.cnki.xdfckjz.2017.11.001
正交设计优化光动力治疗宫颈癌的作用参数
Optimization of photodynamic therapy induced apoptosis of cervical cancer cells by or-thogonal design
摘要
Abstract
Objective:To search the optimum parameters of curcumin as a photosensiti-zer of photodynamic therapy induced apoptosis of human cervical carcinoma Me180 cells by or-thogonal design. Methods:The selected photosensitizer curcumin concentration,laser dosage,ir-radiation time, incubation time four factors, according to the concentration of curcumin 2. 5μmol/L,5μmol/L,10μmol/L,50J/cm2 ,100J/cm2 ,200J/cm2 laser dose,irradiation time:90s, 180s,360s,incubation time:12h,24h,36h. 3 levels of each factor design and orthogonal design table,photodynamic therapy of curcumin by orthogonal design table as a photosensitizer,after treatment of cells,cell survival rate was measured by MTT method. The average marginal value of three levels of curcumin and laser dosage were estimated by two factor analysis of variance. Results:The order of influence factors of cell apoptosis induced by PDT is:curcumin dose >in-cubation time > the laser irradiation time >laser dosage. The level of various factors:Curcumin concentration:10μmol/L>5μmol/L>2. 5μmol/L;200J/cm2>100J/cm2>50J/cm2 laser dose;irradiation time:360s>180s>90s;incubation time:24h>12h>36h. Double factor variance analy-sis showed that curcumin concentration of 2. 5μmol/L,5μmol/L,10μmol/L among the three groups were statistically significant differences(P<0. 05);100J/cm2,200J/cm2 and 50J/cm2 group,there was a statistically significant difference (P<0. 05),100J/cm2 and 200J/cm2 but no statistically significant difference between the groups. Conclusions:Curcumin mediated photo-dynamic therapy induced apoptosis of cervical cancer cells with the best combination of curcu-min concentration of 5μmol/L,laser dose of 100J/cm2 ,irradiation time of 180s,incubation time of 24h.关键词
正交设计/姜黄素/光动力疗法/凋亡/宫颈肿瘤/边际分析Key words
Orthogonal design/Curcumin/Photodynamic therapy/Apoptosis/Cervical cancer/Marginal analysis分类
医药卫生引用本文复制引用
贺桂芳,向青,陈志华,原亚莉,张媛,杨文艳..正交设计优化光动力治疗宫颈癌的作用参数[J].现代妇产科进展,2017,26(11):801-805,5.基金项目
国家自然科学基金(No:81202966) （No:81202966）
