现代妇产科进展2017，Vol.26Issue(11)：806-809,4.DOI:10.13283/j.cnki.xdfckjz.2017.11.036
阿帕替尼对宫颈癌细胞增殖及化疗敏感性的影响
Effect of apatinib on inhibiting proliferation and increasing chemosensitivity of cervical cancer cells
摘要
Abstract
Objective:To investigate the effect of inhibitory porliferation,and chemo-sensitivity of apatinib on human cervical cancer cells. Methods:The effect of porliferation,colo-ny forming ability, cell cycle of apatinib on SiHa and HCC94 cervical cancer cell lines were measured by CCK-8 assay,clone formation test and flow cytometry,respectively. The expression of Cyclin D3 mRNA in cells was examined by RT-qPCR. The expression of Cyclin D3 in cells was detected by Western blot. The effect of chemosensitivity of apatinib to several kinds of an-tineoplastic drug for cervical cancer cell lines were detected by CCK-8 assay. Results:Apatinib inhibited cervical cancer cells growth in a dose-dependent manner, and arrested cell cycle at G0/G1 period. Apatinib can significantly reduce the expressions of Cyclin D3 in a dose-depend-ent manner and can increase the sensitivity to adriamycin and paclitaxel of cervical cancer cells in vitro. Conclusions:Apatinib can inhibit the proliferation, arrest cell cycle at G0/G1 period and enhance chemotherapeutic action of paclitaxel against cervical cancer cells in vitro.关键词
阿帕替尼/宫颈癌/周期/化疗敏感性Key words
Apatinib/Cervical cancer/Cell cycle/Chemosensitivity分类
医药卫生引用本文复制引用
刘秋利,李婧,李娟,齐晓薇,陈玲,唐美,余进进,邱海峰,王元..阿帕替尼对宫颈癌细胞增殖及化疗敏感性的影响[J].现代妇产科进展,2017,26(11):806-809,4.基金项目
江苏省无锡市科技局项目(No:CSE31N1418) （No:CSE31N1418）
无锡市卫生局项目(No:MS201417、FYKY201401) （No:MS201417、FYKY201401）
无锡市医院管理中心科研项目(No:YGZXG1405) （No:YGZXG1405）
国家自然科学基金资助项目(No:81502262) （No:81502262）
河南省博士后科研项目(No:2014021) （No:2014021）
河南省医学科技攻关项目(No:201503045) （No:201503045）
