畜牧兽医学报2017，Vol.48Issue(5)：793-801,9.DOI:10.11843/j.issn.0366-6964.2017.05.002
猪ZP4基因的SNP鉴定、选择压及其与产仔数的关联分析
Study on SNP Identification, Selective Pressure and Association Analysis with Litter Size for ZP4 Gene in Pigs
摘要
Abstract
In this study,we investigated the single nucleotide polymorphisms (SNP) of ZP4 gene,its selective pressure and association with litter size in pigs.Totally,328 sows and their litter size records were collected from Large White,Landrace and Landrace× Large White hybridization pig populations.Direct sequencing method was used to clone and identify the nucleotide mutation in ZP4.The generalized linear regression model was applied to investigate the correlation between ZP4 SNP and litter size.The lositan software,Tajima's testing and Fu and Li's D testing were used to detect the selective pressure of ZP4.Our results demonstrated that 27 SNPs were detected in ZP4,and A268T was significantly correlated with number of total litter size in the 3 populations (P＜ 0.05).A268T was only significantly associated with number of born alive in Landrace and Large White pig populations (P＜0.05).We also detected that T2950C was under positive selection and this mutation was linked with A268T.Our results suggested that A268T was a direct selection marker that could be used in pig marker assistant selection.关键词
ZP4/SNP/选择压/关联分析/产仔数Key words
ZP4/SNP/selective pressure/association analysis/litter size分类
农业科技引用本文复制引用
梁国明,陈锋剑,赵勤涛,刘辰晖,唐中林..猪ZP4基因的SNP鉴定、选择压及其与产仔数的关联分析[J].畜牧兽医学报,2017,48(5):793-801,9.基金项目
国家科技支撑项目(2015BAD03B02) （2015BAD03B02）
