畜牧兽医学报2017，Vol.48Issue(5)：802-809,8.DOI:10.11843/j.issn.0366-6964.2017.05.003
腺病毒介导的MAT2B过表达促进猪肌内脂肪细胞分化
MAT2B Overexprssion Mediated by Adenovirus Promoting Porcine Intramuscular Preadipocyte Differentiation
摘要
Abstract
The aim of this study was to explore the effect of MAT2B on porcine intramuscular preadipocyte differentiation by constructing overexpression vector in vitro mediated by adenovirus.A pair of exclusive primers was designed according to the GenBank sequence information of MAT2B gene,and MAT2B was amplified by PCR.The obtained PCR products were inserted into a shuttle vector pAdTrack-CMV,then the gene was identified by sequencing.The results showed that shuttle vector (pAdTrack-CMV) and backbone vector (pAdEasy-1) were in homologous recombination,and the adenovirus recombinant vector (pAd-MAT2B) was constructed successfully.Then the recombinant adenovirus DNA was digested by the Pac I,the pAd-MAT2B adenovirus was produced,and we used it to infect the porcine preadipocytes.The expression level of mRNA and protein of MAT2B significantly increased.Oil Red O staining assay showed that overexpression of MAT2B promoted lipid accumulation in intramuscular preadipocyte.Moreover,the expression of adipogenic gene PPARγ and aP2 mRNA were significantly promoted when infected by Ad-MAT2B.The results suggest that MAT2B is overexpressed in expression vector in vitro mediated by adenovirus,and MAT2B play a positive role during porcine intramuscular preadipocyte differentiation.关键词
腺苷甲硫氨酸转移酶2B/重组腺病毒/肌内脂肪细胞/猪Key words
Methionine adenosyltransferase 2B/recombinant adenovirus/intramuscular preadipocyte/pig分类
农业科技引用本文复制引用
赵存真,李黄琨,马云,杨公社..腺病毒介导的MAT2B过表达促进猪肌内脂肪细胞分化[J].畜牧兽医学报,2017,48(5):802-809,8.基金项目
信阳农林学院青年基金项目(201601005) （201601005）
国家重点基础研究发展计划(2012CB124705) （2012CB124705）
